Abstract
In peptides, steady-state fluorescence can be used to measure the intrinsic fluorescence of Trp, Tyr, and Phe residues, as well as associated dyes, which can change upon exposure to different environmental conditions. This technique can be thus used to detect changes in the conformational states of peptides. Time-resolved fluorescence can also be used to study fast motions of peptides and their interactions with fluorescence dyes. Data from several low-resolution spectroscopic techniques, including fluorescence, can be combined to generate an overall picture of peptide structure as a function of environmental conditions.
Mangala R. Liyanage and Kunal Bakshi have contributed equally to this chapter.
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References
Lakowicz JR (2006) Principles of fluorescence spectroscopy, 3rd edn. Kluwer, New York
Wiechelman KJ (1986) Empirical correction equation for the fluorescence inner filter effect. Am Lab 18:49–53
Kubista M, Sjobakc R, Erksson S, Albinsson B (1994) Experimental correction for the inner-filter effect in fluorescence spectra. Analyst 119:417–419
Birch DJS, Imhof RE (1991) Time-domain fluorescence spectroscopy using time-correlated single-photon counting. In: Lakowicz JR (ed) Topics in fluorescence spectroscopy, vol 1, Techniques. Plenum, New York, pp 1–95
Lakowicz JR, Gryczynski I (1991) Frequency-domain fluorescence spectroscopy. In: Lakowicz JR (ed) Topics in fluorescence spectroscopy, vol 1, Techniques. Plenum, New York, pp 293–355
O’Connor DV, Phillips D (1984) Time-correlated single photon counting. Academic, London
Unruh JR, Liyanage MR, Johnson CK (2007) Tyrosyl rotamer interconversion rates and the fluorescence decays of n-acetyltyrosinamide and short tyrosyl peptides. J Phys Chem B 111:5494–5502
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Liyanage, M.R., Bakshi, K., Volkin, D.B., Middaugh, C.R. (2014). Fluorescence Spectroscopy of Peptides. In: Nixon, A. (eds) Therapeutic Peptides. Methods in Molecular Biology, vol 1088. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-673-3_16
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DOI: https://doi.org/10.1007/978-1-62703-673-3_16
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