Abstract
MLST is a widely accepted method of sequence-based typing that relies on analysis of relatively conserved genes that encode essential proteins. For Staphylococcus aureus the level of discrimination provided by MLST is sufficient to provide a relatively detailed picture of the global dissemination of the pathogen. The method is not restrictive in the precise methodology used to acquire the sequences, but the method of assigning types requires that the data be of high quality. Excellent web-based tools have been developed and are curated by the groups that launched MLST. These tools have allowed the scheme to be maintained as a coherent global asset and assist users in the analysis of their data.
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References
Enright MC, Day NP, Davies CE et al (2000) Multilocus sequence typing for characterization of methicillin-resistant and methicillin-susceptible clones of Staphylococcus aureus. J Clin Microbiol 38:1008–1015
Enright MC, Spratt BG (1999) Multilocus sequence typing. Trends Microbiol 7:482–487
Aanensen DM, Spratt BG (2005) The multilocus sequence typing network: mlst.net. Nucleic Acids Res 33:W728–W733
Ito T, Katayama Y, Asada K et al (2001) Structural comparison of three types of staphylococcal cassette chromosome mec integrated in the chromosome in methicillin-resistant Staphylococcus aureus. Antimicrob Agents Chemother 45:1323–1336
Oliveira DC, de Lencastre H (2002) Multiplex PCR strategy for rapid identification of structural types and variants of the mec element in methicillin-resistant Staphylococcus aureus. Antimicrob Agents Chemother 46:2155–2161
Howe RA, Monk A, Wootton M et al (2004) Vancomycin susceptibility within methicillin-resistant Staphylococcus aureus lineages. Emerg Infect Dis 10:855–857
Feil EJ, Enright MC (2004) Analyses of clonality and the evolution of bacterial pathogens. Curr Opin Microbiol 7:308–313
Enright MC, Robinson DA, Randle G et al (2002) The evolutionary history of methicillin-resistant Staphylococcus aureus (MRSA). Proc Natl Acad Sci USA 99:7687–7692
Robinson DA, Enright MC (2004) Evolution of Staphylococcus aureus by large chromosomal replacements. J Bacteriol 186:1060–1064
Robinson DA, Enright MC (2004) Multilocus sequence typing and the evolution of methicillin-resistant Staphylococcus aureus. Clin Microbiol Infect 10:92–97
Nübel U, Roumagnac P, Feldkamp M et al (2008) Frequent emergence and limited geographic dispersal of methicillin-resistant Staphylococcus aureus. Proc Natl Acad Sci USA 105:14130–14135
Monecke S, Coombs G, Shore AC et al (2011) A field guide to pandemic, epidemic and sporadic clones of methicillin-resistant Staphylococcus aureus. PLoS One 6(4):e17936
Rolo J, Miragaia M, Turlej-Rogacka A et al (2012) High genetic diversity among community-associated staphylococcus aureus in Europe: results from a multicenter study. PLoS One 7(4):e34768
Lamers RP, Stinnett JW, Muthukrishnan G et al (2011) Evolutionary analyses of staphylococcus aureus identify genetic relationships between nasal carriage and clinical isolates. PLoS One 6(1):e16426
Felsenstein J (1993) PHYLIP (Phylogeny Inference Package) version 3.5c. Distributed by the author. Department of Genetics, University of Washington, Seattle.
Feil EJ, Li BC, Aanensen DM et al (2004) eBURST: inferring patterns of evolutionary descent among clusters of related bacterial genotypes from multilocus sequence typing data. J Bacteriol 186:1518–1530
Salipante SJ, Hall BG (2011) Inadequacies of minimum spanning trees in molecular epidemiology. J Clin Microbiol 49:3568–3575
Cheng L, Connor TR, Aanensen DM et al (2011) Bayesian semi-supervised classification of bacterial samples using MLST databases. BMC Bioinformatics 12:302
Day NPJ, Moore CE, Enright MC et al (2001) A link between virulence and ecological abundance in natural populations of Staphylococcus aureus. Science 292:114–116
Day NPJ, Moore CE, Enright MC et al (2002) Retraction of Day et al., Science 292 (5514) 114–116. Science 295:971
Enright MC, Knox K, Griffiths D et al (2000) Molecular typing of bacteria directly from cerebrospinal fluid. Eur J Clin Microbiol Infect Dis 19:627–630
Birtles A, Hardy K, Gray SJ et al (2005) Multilocus sequence typing of Neisseria meningitidis directly from clinical samples and application of the method to the investigation of meningococcal disease case clusters. J Clin Microbiol 43:6007–6014
van Leeuwen WB, Jay C, Snijders S et al (2003) Multilocus sequence typing of Staphylococcus aureus with DNA array technology. J Clin Microbiol 41:3323–3326
Singh P, Foley SL, Nayak R et al (2012) Multilocus sequence typing of Salmonella strains by high-throughput sequencing of selectively amplified target genes. J Microbiol Methods 88:127–133
Boers SA, van der Reijden WA, Jansen R (2012) High-throughput multilocus sequence typing: bringing molecular typing to the next level. PLoS One 7(7):e39630
Verghese B, Schwalm ND, Dudley EG et al (2012) A combined multi-virulence-locus sequence typing and Staphylococcal Cassette Chromosome mec typing scheme possesses enhanced discriminatory power for genotyping MRSA. Infect Genet Evol 12:1816–1821
Hall TA (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 41:95–98
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The authors acknowledge the support of the Health Protection Agency.
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Saunders, N.A., Holmes, A. (2014). Multilocus Sequence Typing (MLST) of Staphylococcus aureus . In: Ji, Y. (eds) Methicillin-Resistant Staphylococcus Aureus (MRSA) Protocols. Methods in Molecular Biology, vol 1085. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-664-1_7
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DOI: https://doi.org/10.1007/978-1-62703-664-1_7
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