Abstract
Plant organs and tissues consist of many various cell types, often in different phases of their development. Such complex structures do not allow direct studies on behavior of individual cells. In contrast, populations of in vitro-cultured plant cells represent valuable tool for studying processes on a single-cell level, including cell morphogenesis. Here we describe characteristics of well-established model tobacco and Arabidopsis cell lines and provide detailed protocol on their cultivation, characterization, and genetic transformation.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Similar content being viewed by others
References
Tulecke W, Nickell LG (1959) Production of large amounts of plant tissue by submerged culture. Science 130:863–864
Nickell LG, Tulecke W (1960) Submerged growth of cells of higher plants. Biotechnol Bioeng 2:287–297
Street HE, King PJ, Mansfield KJ (1971) Growth control in plant cell suspension cultures. In: Les cultures de tissus de plants. Colloques Internationaux du C.N.R.S. 193. Éditions du Centre National de la Recherche Scientifique, Paris, p 2–40
Opatrný Z (1971) Using of tissue cultures in plant genetics. Thesis, Inst. Exp. Bot. ČSAV, Prague (in Czech)
Opatrný Z, Opatrná J (1976) The specificity of the effect of 2,4-D and NAA on the growth, micromorphology, and occurrence of starch in long-term Nicotiana tabacum L. cell strains. Biol Plantarum 18:359–365
Nagata T, Nemoto Y, Hasezawa S (1992) Tobacco BY-2 cell line as the “HeLa” cell in the cell biology of higher plants. Int Rev Cytol 132:1–30
Nagata T, Hasezawa S, Inzé D (eds) (2004) Tobacco BY-2 cells. Biotechnology in agriculture and forestry, vol 53. Springer, Berlin, Heidelberg
Nagata T, Matsuoka K, Inzé D (eds) (2006) Tobacco BY-2 cells: from cellular dynamics to omics. Biotechnology in agriculture and forestry, vol 58. Springer, Berlin, Heidelberg
Petrášek J, Freudereich A, Heuing A et al (1998) Heat-shock protein 90 is associated with microtubules in tobacco cells. Protoplasma 202:161–174
Campanoni P, Blasius B, Nick P (2003) Auxin transport synchronizes the pattern of cell division in a tobacco cell line. Plant Physiol 133: 1251–1260
Holweg C, Honsel A, Nick P (2003) A myosin inhibitor impairs auxin-induced cell division. Protoplasma 222:193–204
Campanoni P, Nick P (2005) Auxin-dependent cell division and elongation. 1-naphtaleneacetic acid and 2,4-dichlorophenoxyacetic acid activate different pathways. Plant Physiol 137: 939–948
Paciorek T, Zažímalová E, Ruthardt N et al (2005) Auxin inhibits endocytosis and promotes its own efflux from cells. Nature 435:1251–1256
Qiao F, Petrášek J, Nick P (2010) Light can rescue auxin-dependent synchrony of cell division in a tobacco cell line. J Exp Bot 61:503–510
Marhavý P, Bielach A, Abas L et al (2011) Cytokinin modulates endocytotic trafficking of PIN1 auxin efflux carrier to control plant organogenesis. Dev Cell 21:796–804
Kovařík A, Lim K-Y, Součková-Skalická K et al (2012) A plant culture (BY-2) widely used in molecular and cell studies is genetically unstable and highly heterogeneous. Bot J Linn Soc 170:459–471
May MJ, Leaver CJ (1993) Oxidative stimulation of glutathione synthesis in Arabidopsis thaliana suspension cultures. Plant Physiol 103:621–627
Matsunaga S, Ohmido N, Fukui K (2006) Chromosome dynamics in tobacco BY-2 cultured cell. In: Nagata T, Matsuoka K, Inzé D (eds) Tobacco BY-2 cells: from cellular dynamics to omics. Biotechnology in agriculture and forestry, vol 58. Springer, Berlin, Heidelberg, pp 51–63
Frey N, Klotz J, Nick P (2010) A kinesin with calponin-homology domain is involved in premitotic nuclear migration. J Exp Bot 61: 3423–3437
Widholm JM (1972) The use of fluorescein diacetate and phenosafranine for determining viability of cultured plant cells. Stain Technol 47:189–194
Acknowledgments
The authors acknowledge support for their work by the Grant Agency of the Czech Republic (projects P305/11/0797 and P305/11/2476), Ministry of Education, Youth and Sport of the Czech Republic (project MSM00216208858), and Charles University in Prague (project SVV 265203/2012).
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2014 Springer Science+Business Media, New York
About this protocol
Cite this protocol
Seifertová, D., Klíma, P., Pařezová, M., Petrášek, J., Zažímalová, E., Opatrný, Z. (2014). Plant Cell Lines in Cell Morphogenesis Research. In: Žárský, V., Cvrčková, F. (eds) Plant Cell Morphogenesis. Methods in Molecular Biology, vol 1080. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-643-6_18
Download citation
DOI: https://doi.org/10.1007/978-1-62703-643-6_18
Published:
Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-62703-642-9
Online ISBN: 978-1-62703-643-6
eBook Packages: Springer Protocols