Abstract
The availability of mesenchymal stem cells (MSCs) or skeletal stem cells (SSCs) is vital to many of the tissue engineering strategies currently being developed for repairing bone and cartilage. One difficulty with using this cell population is that SSCs represent only a small fraction of the cells available from an individual patient’s bone marrow sample, typically less than 1 in 10,000. Therefore, methods have been devised to enrich the proportion of MSCs obtained from a bone marrow sample using hybridoma cell lines to generate antibodies to cell surface antigens specific for MSCs. Stro-1 is the most widely targeted of these cell surface antigens. The protocol described overleaf is used to isolate and enrich the Stro-1 positive fraction of cells from a bone marrow aspirate to provide a sample enriched for MSCs for use in both in vitro and in vivo studies.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Similar content being viewed by others
References
Kolf CM, Cho E, Tuan RS (2007) Mesenchymal stromal cells. Biology of adult mesenchymal stem cells: regulation of niche, self-renewal and differentiation. Arthritis Res Ther 9(1):204, Epub 2007/02/24
Simmons PJ, Torok-Storb B (1991) Identification of stromal cell precursors in human bone marrow by a novel monoclonal antibody, STRO-1. Blood 78(1):55–62, Epub 1991/07/01
Stewart K, Walsh S, Screen J, Jefferiss CM, Chainey J, Jordan GR et al (1999) Further characterization of cells expressing STRO-1 in cultures of adult human bone marrow stromal cells. J Bone Miner Res 14(8):1345–1356, Epub 1999/08/24
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2013 Springer Science+Business Media, LLC
About this protocol
Cite this protocol
Williams, E.L., White, K., Oreffo, R.O.C. (2013). Isolation and Enrichment of Stro-1 Immunoselected Mesenchymal Stem Cells from Adult Human Bone Marrow. In: Turksen, K. (eds) Stem Cell Niche. Methods in Molecular Biology, vol 1035. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-508-8_7
Download citation
DOI: https://doi.org/10.1007/978-1-62703-508-8_7
Published:
Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-62703-507-1
Online ISBN: 978-1-62703-508-8
eBook Packages: Springer Protocols