Skip to main content
SpringerLink
Log in

Mouse Model of Invasive Fungal Infection

  • Protocol
  • First Online:
Mouse Models of Innate Immunity

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1031))

Abstract

The mouse intravenous (IV) challenge model of Candida albicans invasive fungal infection has been widely used to study the importance of the innate immune system in these infections. This chapter describes this well-characterized model, where fungal cells are administered directly into the mouse bloodstream to initiate a systemic infection. The preparation of tissue samples from infected mice to allow evaluation of disease progression and host responses is also described.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution

Subscribe and save

Springer+ Basic
$34.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or eBook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Protocol
USD 49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 119.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Similar content being viewed by others

References

  1. Kontoyiannis DP, Marr KA, Park BJ et al (2010) Prospective surveillance for invasive fungal infections in hematopoietic stem cell transplant recipients, 2001–2006: overview of the transplant-associated infection surveillance network (TRANSNET) database. Clin Infect Dis 50:1091–1100

    Article  PubMed  Google Scholar 

  2. Pappas PG, Alexander BD, Andes DR et al (2010) Invasive fungal infections among organ transplant recipients: results of the transplant-associated infection surveillance network (TRANSNET). Clin Infect Dis 50:1101–1111

    Article  PubMed  Google Scholar 

  3. Holley A, Dulhunty J, Blot S, Lipman J, Lobo S, Dancer C, Rello J, Dimopoulos G (2009) Temporal trends, risk factors and outcomes in albicans and non-albicans candidaemia: an international epidemiological study in four multidisciplinary intensive care units. Int J Antimicrob Agents 33:554.e1–554.e7

    Article  Google Scholar 

  4. Horn DL, Neofytos D, Anaissie EJ, Fishman JA, Steinbach WJ, Olyaei AJ, Marr KA, Pfaller MA, Chang CH, Webster KM (2009) Epidemiology and outcomes of candidemia in 2019 patients: data from the prospective antifungal therapy alliance registry. Clin Infect Dis 48:1695–1703

    Article  PubMed  CAS  Google Scholar 

  5. MacCallum DM (2010) Candida infections and modelling disease. In: Ashbee HR, Bignell E (eds) Pathogenic yeasts, the yeast handbook. Springer, New York, pp 41–67

    Chapter  Google Scholar 

  6. Graf K, Khani SM, Ott E, Mattner F, Gastmeier P, Sohr D, Ziesing S, Chaberny IF (2011) Five-years surveillance of invasive aspergillosis in a university hospital. BMC Infect Dis 11:163

    Article  PubMed  Google Scholar 

  7. Latge JP (1999) Aspergillus fumigatus and aspergillosis. Clin Microbiol Rev 12:310–350

    PubMed  CAS  Google Scholar 

  8. Chamilos G, Luna M, Lewis RE, Bodey GP, Chemaly R, Tarrand JJ, Safdar A, Raad II, Kontoyiannis DP (2006) Invasive fungal infections in patients with hematologic malignancies in a tertiary care cancer center: an autopsy study over a 15-year period (1989–2003). Haematologica 91:986–989

    PubMed  Google Scholar 

  9. Anane S, Khalfallah F (2007) Biological diagnosis of systemic candidiasis: difficulties and future prospects. Pathol Biol 55:262–272

    Article  PubMed  CAS  Google Scholar 

  10. Cuenca-Estrella M, Bassetti M, Lass-Florl C, Racil Z, Richardson M, Rogers TR (2011) Detection and investigation of invasive mould disease. J Antimicrob Chemother 66(Suppl 1):i15–i24

    Article  PubMed  CAS  Google Scholar 

  11. Morace G, Borghi E (2010) Fungal infections in ICU patients: epidemiology and the role of diagnostics. Minerva Anestesiol 76:950–956

    PubMed  CAS  Google Scholar 

  12. MacCallum DM (2012) Mouse intravenous challenge models and applications. Meth Mol Biol 845:499–509

    Article  CAS  Google Scholar 

  13. Szabo EK, MacCallum DM (2011) The contribution of mouse models to our understanding of systemic candidiasis. FEMS Microbiol Lett 320:1–8

    Article  PubMed  CAS  Google Scholar 

  14. MacCallum DM, Odds FC (2005) Temporal events in the intravenous challenge model for experimental Candida albicans infections in female mice. Mycoses 48:151–161

    Article  PubMed  Google Scholar 

  15. Louria DB, Brayton RG, Finkel G (1963) Studies on the pathogenesis of experimental Candida albicans infections in mice. Sabouraudia 2:271–283

    Article  Google Scholar 

  16. Lionakis MS, Lim JK, Lee CC, Murphy PM (2011) Organ-specific innate immune responses in a mouse model of invasive candidiasis. J Innate Immun 3:180–199

    Article  PubMed  CAS  Google Scholar 

  17. Spellberg B, Johnston D, Phan QT, Edwards JE Jr, French SW, Ibrahim AS, Filler SG (2003) Parenchymal organ, and not splenic, immunity correlates with host survival during disseminated candidiasis. Infect Immun 71:5756–5764

    Article  PubMed  CAS  Google Scholar 

  18. MacCallum DM (2009) Massive induction of innate immune response to Candida albicans in the kidney in a murine intravenous challenge model. FEMS Yeast Res 9:1111–1122

    Article  PubMed  CAS  Google Scholar 

  19. Spellberg B, Ibrahim AS, Edwards JE Jr, Filler SG (2005) Mice with disseminated candidiasis die of progressive sepsis. J Infect Dis 192:336–343

    Article  PubMed  Google Scholar 

  20. MacCallum DM, Castillo L, Brown AJP, Gow NAR, Odds FC (2009) Early-expressed chemokines predict kidney immunopathology in experimental disseminated Candida albicans infections. PLoS One 4:e6420

    Article  PubMed  Google Scholar 

  21. MacCallum DM, Castillo L, Nather K, Munro CA, Brown AJ, Gow NA, Odds FC (2009) Property differences among the four major Candida albicans strain clades. Eukaryot Cell 8:373–387

    Article  PubMed  CAS  Google Scholar 

  22. Asmundsdottir LR, Erlendsdottir H, Agnarsson BA, Gottfredsson M (2009) The importance of strain variation in virulence of Candida dubliniensis and Candida albicans: results of a blinded histopathological study of invasive candidiasis. Clin Microbiol Infect 15:576–585

    Article  PubMed  CAS  Google Scholar 

  23. Selmecki A, Bergmann S, Berman J (2005) Comparative genome hybridization reveals widespread aneuploidy in Candida albicans laboratory strains. Mol Microbiol 55:1553–1565

    Article  PubMed  CAS  Google Scholar 

  24. Odds FC, Van Nuffel L, Gow NA (2000) Survival in experimental Candida albicans infections depends on inoculum growth conditions as well as animal host. Microbiology 146:1881–1889

    PubMed  CAS  Google Scholar 

  25. Hector RF, Domer JE, Carrow EW (1982) Immune responses to Candida albicans in genetically distinct mice. Infect Immun 38:1020–1028

    PubMed  CAS  Google Scholar 

  26. Marquis G, Montplaisir S, Pelletier M, Mousseau S, Auger P (1986) Strain-dependent differences in susceptibility of mice to experimental candidosis. J Infect Dis 154:906–909

    Article  PubMed  CAS  Google Scholar 

  27. Ashman RB, Fulurija A, Papadimitriou JM (1996) Strain-dependent differences in host response to Candida albicans infection in mice are related to organ susceptibility and infectious load. Infect Immun 64:1866–1869

    PubMed  CAS  Google Scholar 

  28. Murciano C, Yanez A, O’Connor JE, Gozalbo D, Gil ML (2008) Influence of aging on murine neutrophil and macrophage function against Candida albicans. FEMS Immunol Med Microbiol 53:214–221

    Article  PubMed  CAS  Google Scholar 

  29. Ashman RB, Papadimitriou JM, Fulurija A (1999) Acute susceptibility of aged mice to infection with Candida albicans. J Med Microbiol 48:1095–1102

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgments

Research in the laboratory of DMM is supported by grants from the Wellcome Trust (089930), EC (STRIFE) and National Centre for the Replacement, Reduction and Refinement of Animals in Research (NC3Rs).

Author information

Authors and Affiliations

  1. Aberdeen Fungal Group, Institute of Medical Sciences, University of Aberdeen, Aberdeen, UK

    Donna M. MacCallum

Authors
  1. Donna M. MacCallum
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. , Dept. of Biomedical Sciences & Pathobiol, Virginia Tech, Duckpond Drive, Blacksburg, 24061, Virginia, USA

    Irving C. Allen

Rights and permissions

Reprints and permissions

Copyright information

© 2013 Springer Science+Business Media, LLC

About this protocol

Cite this protocol

MacCallum, D.M. (2013). Mouse Model of Invasive Fungal Infection. In: Allen, I. (eds) Mouse Models of Innate Immunity. Methods in Molecular Biology, vol 1031. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-481-4_17

Download citation

  • DOI: https://doi.org/10.1007/978-1-62703-481-4_17

  • Published:

  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-480-7

  • Online ISBN: 978-1-62703-481-4

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics

Search

Navigation