Abstract
In this article we provide a method to isolate hair follicle stem cells that have undergone targeted gene inactivation. The mice from which these cells are isolated are bred into a Rosa26-yellow fluorescent protein (YFP) reporter background, which results in YFP expression in the targeted stem cell population. These cells are isolated and purified by fluorescence-activated cell sorting, using epidermal stem cell-specific markers in conjunction with YFP fluorescence. The purified cells can be used for gene expression studies, clonogenic experiments, and biological assays, such as viability and capacity for directional migration.
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Acknowledgments
We thank K. Chadwick for expert help with FACS experiments. This work was supported with funding from the Canadian Institutes of Health Research to L.D.
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Nakrieko, KA., Irvine, T.S., Dagnino, L. (2013). Isolation of Hair Follicle Bulge Stem Cells from YFP-Expressing Reporter Mice. In: Turksen, K. (eds) Skin Stem Cells. Methods in Molecular Biology, vol 989. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-330-5_3
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DOI: https://doi.org/10.1007/978-1-62703-330-5_3
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Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-62703-329-9
Online ISBN: 978-1-62703-330-5
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