Abstract
Several different precursor populations participate in renewal and regeneration of the mammalian skin and hair follicle. Recently, we described the existence of multipotent dermal precursors that exhibit properties of stem cells, and reside in the mesenchymal compartment of the hair follicle. When isolated and grown in vitro, these cells give rise to self-renewing, multipotent, spherical colonies of cells called Skin-derived Precursors (or “SkPs”). Here we describe methods to isolate SkPs from rodent and human skin and provide assays to determine functional differentiation of their progeny.
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References
Blanpain C, Fuchs E (2009) Epidermal homeostasis: a balancing act of stem cells in the skin. Nat Rev Mol Cell Biol 10(3):207–217. doi:nrm2636 [pii] 10.1038/nrm2636
Toma JG, Ahkavan M, Fernandes KJL, Barnabe-Heider F, Sadikot A, Kaplan DR, Miller FD (2001) Isolation of multipotent adult stem cells from the dermis of mammalian skin. Nat Cell Biol 3:778–784
Fernandes KJL, McKenzie IA, Mill P, Smith KM, Akhavan M, Barnabe-Heider F, Biernaskie J, Junek A, Kobayashi NR, Toma JG, Kaplan DR, Labosky PA, Rafuse V, Hui C-C, Miller FD (2004) A dermal niche for mutipotent adult skin-derived precursor cells. Nat Cell Biol 6(11):1082–1093
Biernaskie J, Paris M, Morozova O, Fagan BM, Marra M, Pevny L, Miller FD (2009) SKPs derive from hair follicle precursors and exhibit properties of adult dermal stem cells. Cell Stem Cell 5(6):610–623. doi:S1934-5909(09)00573-6 [pii] 10.1016/j.stem.2009.10.019
Pearse DD, Pereira FC, Marcillo AE, Bates ML, Berrocal YA, Filbin MT, Bunge MB (2004) cAMP and Schwann cells promote axonal growth and functional recovery after spinal cord injury. Nat Med 10(6):610–616
Xu XM, Chen A, Guenard V, Kleitman N, Bunge MB (1997) Bridging Schwann cell transplants promote axonal regeneration from both the rostral and caudal stumps of transected adult rat spinal cord. J Neurocytol 26(1):1–16
Pinzon A, Calancie B, Oudega M, Noga BR (2001) Conduction of impulses by axons regenerated in a Schwann cell graft in the transected adult rat thoracic spinal cord. J Neurosci Res 64(5):533–541
Paino CL, Bunge MB (1991) Induction of axon growth into Schwann cell implants grafted into lesioned adult rat spinal cord. Exp Neurol 114(2):254–257
Barakat DJ, Gaglani SM, Neravetla SR, Sanchez AR, Andrade CM, Pressman Y, Puzis R, Garg MS, Bunge MB, Pearse DD (2005) Survival, integration, and axon growth support of glia transplanted into the chronically contused spinal cord. Cell Transplant 14(4):225–240
McKenzie IA, Biernaskie J, Toma JG, Midha R, Miller FD (2006) Skin-derived precursors generate myelinating Schwann cells for the injured and dysmyelinated nervous system. J Neurosci 26(24):6651–6660
Biernaskie J, Sparling JS, Liu J, Shannon CP, Plemel JR, Xie Y, Miller FD, Tetzlaff W (2007) Skin-derived precursors generate myelinating Schwann cells that promote remyelination and functional recovery after contusion spinal cord injury. J Neurosci 27(36):9545–9559. doi:27/36/9545 [pii] 10.1523/JNEUROSCI.1930-07.2007
Lichti U, Weinberg WC, Goodman L, Ledbetter S, Dooley T, Morgan D, Yuspa SH (1993) In vivo regulation of murine hair growth: insights from grafting defined cell populations onto nude mice. J Invest Dermatol 101(1 Suppl):124S–129S
Zheng Y, Du X, Wang W, Boucher M, Parimoo S, Stenn K (2005) Organogenesis from dissociated cells: generation of mature cycling hair follicles from skin-derived cells. J Invest Dermatol 124(5):867–876
Acknowledgments
We thank Mr. Ranjan Kumar for his assistance with images. This work was funded by a CIHR operating grant and a CIHR New Investigator Award to J.B.
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Hagner, A., Biernaskie, J. (2013). Isolation and Differentiation of Hair Follicle-Derived Dermal Precursors. In: Turksen, K. (eds) Skin Stem Cells. Methods in Molecular Biology, vol 989. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-330-5_19
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DOI: https://doi.org/10.1007/978-1-62703-330-5_19
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Publisher Name: Humana Press, Totowa, NJ
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