Abstract
The identification of novel substrates of cytochrome P450 enzymes by high-throughput screening assays is of utmost importance to further increase the scope of these enzymes for future applications. Most screens are either confined to individual substrate analogues or hampered by low throughput due to elaborate analysis techniques. Here we describe a general high-throughput screening assay that interrogates the activity of P450 enzymes with the aid of catalase as a reporter enzyme.
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Acknowledgments
We are most grateful to Deutsche Forschungsgemeinschaft (DFG), Bundesministerium für Bildung und Forschung (BMBF), Alexander-von-Humboldt Stiftung, the European Union, and the Max-Planck Society for financial support of our work. KSR thanks the Deutscher Akademischer Austauschdienst (DAAD) for a postdoctoral fellowship.
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Rabe, K.S., Niemeyer, C.M. (2013). Screening for Cytochrome P450 Reactivity with a Reporter Enzyme. In: Phillips, I., Shephard, E., Ortiz de Montellano, P. (eds) Cytochrome P450 Protocols. Methods in Molecular Biology, vol 987. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-321-3_13
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DOI: https://doi.org/10.1007/978-1-62703-321-3_13
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Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-62703-320-6
Online ISBN: 978-1-62703-321-3
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