Abstract
Hematopoietic stem cells (HSCs) are capable of self-renewal and multi-lineage reconstitution of hematopoiesis in irradiated transplant recipient mice. As such, bone marrow transplantation (BMT) is a major assay commonly used to examine murine HSC activity. BMT traditionally involves injection of HSCs into lethally irradiated recipients via the tail vein, then subsequently analyzing donor engraftment. Here, we describe the methods for assaying HSC reconstitution in direct, competitive, and serial BMT.
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Acknowledgments
We thank Dr. Hongmei Shen for her critical review of this manuscript. This work was supported by the grants from the Ministry of Science and Technology of China (2011CB964800) and the National Science Foundation of China (81090410 and 30825017) to TC. TC was a recipient of the Scholar Award from the Leukemia & Lymphoma Society (1027–08).
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Cheng, H., Liang, P.H., Cheng, T. (2013). Mouse Hematopoietic Stem Cell Transplantation. In: Turksen, K. (eds) Stem Cells and Aging. Methods in Molecular Biology, vol 976. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-317-6_3
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DOI: https://doi.org/10.1007/978-1-62703-317-6_3
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Publisher Name: Humana Press, Totowa, NJ
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