Abstract
Tissue microarrays (TMAs) enable high-throughput tissue analysis by selecting a large number of paraffin-embedded donor tissue block cores and transferring these tissue cores into a positionally encoded array in the recipient TMA block. Once TMAs are constructed, a variety of analysis may be performed on the arrays including histochemical, immunohistochemical, or immunofluorescent staining, and in situ hybridization for DNA or RNA. TMAs offer a cost-effective method for performing parallel analysis of a large number of tissue samples. In this chapter we outline the method of TMA construction with an emphasis on providing useful information in the analysis of a variety of pancreatic neoplasms, including pancreatic adenocarcinomas and pre-invasive lesions. The technique of TMA construction in this chapter is restricted to the use of formalin-fixed paraffin-embedded tissue.
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Acknowledgments
The author would like to acknowledge the assistance of Lin Yang, Rina Wu, Ting Ting Wu, and Mariam Amrani, MD, in the preparation of this chapter.
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Remotti, H. (2013). Tissue Microarrays: Construction and Use. In: Su, G. (eds) Pancreatic Cancer. Methods in Molecular Biology, vol 980. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-287-2_2
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DOI: https://doi.org/10.1007/978-1-62703-287-2_2
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