Abstract
Chiral ligand-exchange capillary electrophoresis is a relatively cheap and flexible format of capillary electrophoresis. It separates chiral analytes with a metal complex chiral selector that is composed of a metal ion and either homogeneous or heterogeneous chiral ligands (in respect of analytes). The analytes to be separated are required to possess the ability to coordinate with the metal ion, commonly containing N and/or O atoms, for example, amino acids or amino alcohols. In present chapter we take the separation of dansyl dl-amino acids as a key example to guide through the setup of a chiral ligand-exchange capillary electrophoresis enantioseparation protocol. The critical working conditions emphasized include the selection of metal ion, pH window, the ligand-to-metal ratio and/or the concentrations of the complex and electrolytes. In addition, trouble-shooting advice is given such as the way to overcome baseline drifting.
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References
Lu H, Chen GN (2011) Recent advances of enantioseparations in capillary electrophoresis and capillary electrochromatography. Anal Methods 3:488–508
Schmid MG, Gübitz G (2011) Enantioseparation by chromatographic and electromigration techniques using ligand-exchange as chiral separation principle. Anal Bioanal Chem 400:2305–2316
Davankov VA, Rogozhin SV (1971) Ligand chromatography as a novel method for investigation of mixed complexes-stereoselective effects in alpha-amino acid copper(II) complexse. J Chromatogr 60:280–283
Kurganov A (2001) Chiral chromatographic separations based on ligand exchange. J Chromatogr A 906:51–71
Gassman E, Kuo J, Zare RN (1985) Electrokinetic separation of chiral compounds. Science 230:813–814
Gozel P et al (1987) Electrokinetic resolution of amino acid enantiomers with copper(II)-aspartame support electrolyte. Anal Chem 59:44–49
Hödl H et al (2007) Chiral separation of sympathomimetics and β-blockers by ligand-exchange CE using Cu(II) complexes of L-tartaric acid and L-threonine as chiral selectors. Electrophoresis 28:2675–2682
Kodama S et al (2010) Metal(II)–ligand molar ratio dependence of enantioseparation of tartaric acid by ligand exchange CE with Cu(II) and Ni(II)–D-quinic acid systems. Electrophoresis 31:1051–1054
Rizkov D et al (2010) β-Amino alcohol selectors for enantioselective separation of amino acids by ligand-exchange capillary zone electrophoresis in a low molecular weight organogel. Electrophoresis 31:3921–3927
Zheng ZX, Qu F, Lin JM (2003) Investigation of mixed chiral selectors of different metal ion-L-alanine complex and β-cyclodextrin on the chiral separation of dansyl amino acids with capillary electrophoresis. Chin J Chem 21:1478–1484
Hödl H, Schmid MG, Gübitz G (2008) Chiral separation of amino acids and glycyl dipeptides by chiral ligand-exchange capillary electrophoresis comparing Cu(II), Co(II), Ni(II) and Zn(II) complexes of three different sugar acids. J Chromatogr A 1204:210–218
Qi L et al (2007) Chiral CE of aromatic amino acids by ligand-exchange with zinc(II)-L-lysine complex. Electrophoresis 28:2629–2634
Qi L et al (2007) Assay of aromatic amino acid enantiomers in rice-brewed suspensions by chiral ligand-exchange CE. Electrophoresis 28:4150–4155
Qi L et al (2008) Separation of dansylated amino acid enantiomers by chiral ligand-exchange CE with a zinc(II) L-arginine complex as the selecting system. Electrophoresis 29:4277–4283
Lu XN et al (2002) Chiral separation of underivatized amino acids by ligand-exchange capillary electrophoresis using copper(II)-L-lysine complex as selector. J Chromatogr A 945:249–255
Acknowledgments
The financial support from NSFC (No. 21027003 & 20905068), MOST (No.2007CB714504) and CAS are gratefully acknowledged.
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Chen, Y., Song, L. (2013). Enantioseparation by Chiral Ligand-Exchange Capillary Electrophoresis. In: Scriba, G. (eds) Chiral Separations. Methods in Molecular Biology, vol 970. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-263-6_25
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DOI: https://doi.org/10.1007/978-1-62703-263-6_25
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