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Real-Time Chemical Measurements of Dopamine Release in the Brain

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Dopamine

Part of the book series: Methods in Molecular Biology ((MIMB,volume 964))

Abstract

Rapid changes in extracellular dopamine concentrations in freely moving or anesthetized rats can be detected using fast-scan cyclic voltammetry (FSCV). Background-subtracted FSCV is a real-time electrochemical technique that can monitor neurochemical transmission in the brain on a subsecond timescale, while providing chemical information on the analyte. Also, this voltammetric approach allows for the investigation of the kinetics of release and uptake of molecules in the brain. This chapter describes, completely, how to make these measurements and the properties of FSCV that make it uniquely suitable for performing chemical measurements of dopaminergic neurotransmission in vivo.

These two authors contributed equally to this work.

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Acknowledgments

This work was funded in part by grants from the National Institutes of Health, the National Science Foundation, and NCSU Department of Chemistry. In addition, we gratefully acknowledge our coworkers, past and present, for the studies cited in this review.

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Authors and Affiliations

  1. Department of Chemistry, North Carolina State University, Raleigh, NC, USA

    James G. Roberts, Leyda Z. Lugo-Morales, Philip L. Loziuk & Leslie A. Sombers

Authors
  1. James G. Roberts
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  2. Leyda Z. Lugo-Morales
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  3. Philip L. Loziuk
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  4. Leslie A. Sombers
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Corresponding author

Correspondence to Leslie A. Sombers .

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Editors and Affiliations

  1. Krasnow Institute for Advanced Study, Dept. of Molecular Neuroscience, George Mason University, University Drive 4400, Fairfax, 22030, Virginia, USA

    Nadine Kabbani

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Roberts, J.G., Lugo-Morales, L.Z., Loziuk, P.L., Sombers, L.A. (2013). Real-Time Chemical Measurements of Dopamine Release in the Brain. In: Kabbani, N. (eds) Dopamine. Methods in Molecular Biology, vol 964. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-251-3_16

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  • DOI: https://doi.org/10.1007/978-1-62703-251-3_16

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-250-6

  • Online ISBN: 978-1-62703-251-3

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