Abstract
Characterisation of the phenotype and physiological responsiveness of a neuron and identification of its innervation target(s) are important objectives for neuroscientists. This chapter describes the technique of juxtacellular neuronal labelling and presents procedures for the light and electron microscopic visualisation of labelled neurons and the identification of their neurochemical phenotypes and inputs. For those with little experience in extracellular neuronal recording, a detailed description of the required pieces of equipment and how they are configured is also provided.
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Acknowledgments
The work reported here was supported by the National Health and Medical Research Council of Australia (Project Grants 229907, 480414, 350207). The juxtacellularly labelled neuron in Fig. 5 was filled by Dr. Ann Schreihofer in the laboratory of Dr. Patrice Guyenet. We thank them both for allowing us to use this image. The juxtacellularly labelled relaxin-3-positive neuron in Fig. 6 (panels c and d) was filled by Dr. Sherie Ma in the laboratory of Associate Professor Anthony Verberne.
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Verberne, A.J.M., Llewellyn-Smith, I.J. (2013). Juxtacellular Neuronal Labelling, Physiological Characterization and Phenotypic Identification of Single Neurons In Vivo. In: Pilowsky, P., Farnham, M., Fong, A. (eds) Stimulation and Inhibition of Neurons. Neuromethods, vol 78. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-233-9_10
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DOI: https://doi.org/10.1007/978-1-62703-233-9_10
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