Abstract
The cellular level processes cell division and cell expansion form a crucial level linking regulatory processes at the molecular level to whole plant growth rates and organ size and shape. With the rapid progress in molecular profiling, quantification of cellular activities becomes increasingly important to determine sampling strategies that are most informative to understand the molecular basis for organ and plant level phenotypes. Inversely, to understand phenotypes caused by genetic or environmental perturbations it is crucial to know how the cell division and expansion parameters are affected spatially and temporally. Kinematic analyses provide a powerful and rigorous mathematical framework to quantify cell division and cell expansion rates. In dicotyledonous leaves, these processes are primarily changing over time, resulting in division, expansion, and mature phases of development. Monocotyledonous leaves have a persistent spatial gradient, with an intercalary meristem where division takes place, an expansion zone, and a mature part of the leaf. Here we describe in detail how to perform kinematic analyses in leaves of the model species Arabidopsis thaliana and in the leaves of the monocotyledonous crop species Zea mays. These methods can readily be used and adapted to suit other species using relatively standard equipment present in most laboratories. Importantly, the obtained results can be used to design sampling techniques for proliferating, expanding and mature cells.
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References
Poorter H, Garnier E (1996) Plant growth analysis: an evaluation of experimental design and computational methods. J Exp Bot 47(302):1343–1351
Goodwin RH, Stepka W (1945) Growth and differentiation in the root tip of Phleum pratense. Am J Bot 32:36–46
Erickson RO, Sax KB (1956) Rates of cell division and cell elongation in the growth of the primary root of Zea mays. Proc Am Philos Soc 100(5):499–514
Walter A, Silk WK, Schurr U (2009) Environmental effects on spatial and temporal patterns of leaf and root growth. Annu Rev Plant Biol 60:279–304
Fiorani F, Beemster GTS (2006) Quantitative analyses of cell division in plants. Plant Mol Biol 60(6):963–979
Candela H, Martínez-Laborda A, Micol JL (1999) Venation pattern formation in Arabidopsis thaliana vegetative leaves. Dev Biol 205(1):205–216
Donnelly PM et al (1999) Cell cycling and cell enlargement in developing leaves of Arabidopsis. Dev Biol 215:407–419
Ben-Haj-Salah H, Tardieu F (1995) Temperature affects expansion rate of maize leaves without change in spatial distribution of cell length. Analysis of the coordination between cell division and cell expansion. Plant Physiol 109:861–870
Beemster GTS et al (2005) Genome-wide analysis of gene expression profiles associated with cell cycle transitions in growing organs of Arabidopsis. Plant Physiol 138(2):734–743
Beemster GTS, Baskin TI (1998) Analysis of cell division and elongation underlying the developmental acceleration of root growth in Arabidopsis thaliana. Plant Physiol 116(4): 515–526
Basu P et al (2007) A novel image-analysis technique for kinematic study of growth and curvature. Plant Physiol 145(2):305–316
van der Weele CM et al (2003) A new algorithm for computational image analysis of deformable motion at high spatial and temporal resolution applied to root growth. Roughly uniform elongation in the meristem and also, after an abrupt acceleration, in the elongation zone. Plant Physiol 132(3):1138–1148
Granier C, Tardieu F (2009) Multi-scale phenotyping of leaf expansion in response to environmental changes: the whole is more than the sum of parts. Plant Cell Environ 32: 1175–1184
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Nelissen, H., Rymen, B., Coppens, F., Dhondt, S., Fiorani, F., Beemster, G.T.S. (2013). Kinematic Analysis of Cell Division in Leaves of Mono- and Dicotyledonous Species: A Basis for Understanding Growth and Developing Refined Molecular Sampling Strategies. In: De Smet, I. (eds) Plant Organogenesis. Methods in Molecular Biology, vol 959. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-221-6_17
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DOI: https://doi.org/10.1007/978-1-62703-221-6_17
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Publisher Name: Humana Press, Totowa, NJ
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Online ISBN: 978-1-62703-221-6
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