Abstract
Identification of antigenic peptides recognized by cytolytic T lymphocytes (CTL) is a prerequisite for the development of targeted cancer immunotherapy approaches. This chapter provides a global approach for the identification of peptides recognized by CTL. It implies the identification of the HLA molecule presenting the peptide as well as the design and screening of a cDNA library derived from the tumor cells.
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Acknowledgments
We thank J. Klein for editorial assistance. N.V. is a post-doctoral fellow at the Fonds National de la Recherche Scientifique (F.R.S.-F.N.R.S), Belgium and A.M. is supported by a fellowship from the F.R.S.-F.N.R.S (TELEVIE grant No. 7.4592.10).
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Vigneron, N., Ma, W., Michaux, A., Van den Eynde, B.J. (2013). Identifying Source Proteins for MHC Class I-Presented Peptides. In: van Endert, P. (eds) Antigen Processing. Methods in Molecular Biology™, vol 960. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-218-6_16
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DOI: https://doi.org/10.1007/978-1-62703-218-6_16
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