Abstract
Enzyme-linked immunosorbent assays (ELISA) are being used extensively for the identification of Bt-protein in Bt transgenic crops. A sandwich ELISA test is the most preferable immunoassay for the quantification of Bt-protein in transgenic cotton plants. Here, we describe development of sandwich ELISA, employing polyclonal rabbit antibody as a capture antibody and HRP-labeled mouse anti-Bt protein-antibody as a detector antibody.
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Kamle, S., Ojha, A., Kumar, A. (2013). Development of Enzyme-Linked Immunosorbent Assay for the Detection of Bt Protein in Transgenic Cotton. In: Zhang, B. (eds) Transgenic Cotton. Methods in Molecular Biology, vol 958. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-212-4_10
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DOI: https://doi.org/10.1007/978-1-62703-212-4_10
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Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-62703-211-7
Online ISBN: 978-1-62703-212-4
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