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Histochemical and Molecular Quantification of Arbuscular Mycorrhiza Symbiosis

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Plant and Food Carotenoids

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2083))

Abstract

Arbuscular mycorrhizae (AM) are one of the most widespread and studied plant associations with beneficial microorganisms. Indeed, more than 80% of land plants, including most agricultural and horticultural crop species, are able to establish this mutualistic symbiosis with AM fungi. Through this association the fungus helps the plant in the acquisition of water and mineral nutrients, especially under stress conditions. AM symbiosis affects other ecologically and economically important traits such as plant architecture, flowering, and fruit quality but also tolerance against biotic and abiotic stresses. As a consequence, AM fungi have a great potential as biofertilizers and bioprotection agents in sustainable agriculture. However, in order to take advantage of all these benefits, a good and functional symbiosis is required. Here we present methods for reliable quantification of colonization levels which should be useful not only for research but also from the agronomic point of view.

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References

  1. Barea JM, Pozo MJ, Azcon R, Azcon-Aguilar C (2005) Microbial co-operation in the rhizosphere. J Exp Bot 56:1761–1778

    Article  CAS  Google Scholar 

  2. Gianinazzi S et al (2010) Agroecology: the key role of arbuscular mycorrhizas in ecosystem services. Mycorrhiza 20:519–530

    Article  Google Scholar 

  3. Tkacz A, Poole P (2015) Role of root microbiota in plant productivity. J Exp Bot 66:2167–2175

    Article  CAS  Google Scholar 

  4. Pozo MJ, López-Ráez JA, Azcón C, García-Garrido JM (2015) Phytohormones as integrators of environmental signals in the regulation of mycorrhizal symbioses. New Phytol 205:1431–1436

    Article  CAS  Google Scholar 

  5. Duhamel M, Vandenkoornhuyse P (2013) Sustainable agriculture: possible trajectories from mutualistic symbiosis and plant neodomestication. Trends Plant Sci 18:597–600

    Article  CAS  Google Scholar 

  6. López-Ráez JA et al (2015) Differential spatio-temporal expression of carotenoid cleavage dioxygenases regulates apocarotenoid fluxes during AM symbiosis. Plant Sci 230:59–69

    Article  Google Scholar 

  7. Walter MH, Stauder R, Tissier A (2015) Evolution of root-specific carotenoid precursor pathways for apocarotenoid signal biogenesis. Plant Sci 233:1–10

    Article  CAS  Google Scholar 

  8. López-Ráez JA, Shirasu K, Foo E (2017) Strigolactones in plant interactions with beneficial and detrimental organisms: the yin and yang. Trends Plant Sci 22:527–537

    Article  Google Scholar 

  9. Waters MT, Gutjahr C, Bennett T, Nelson DC (2017) Strigolactone signaling and evolution. Annu Rev Plant Biol 68:291–322

    Article  CAS  Google Scholar 

  10. Wang M et al (2018) Blumenols as shoot markers of root symbiosis with arbuscular mycorrhizal fungi. Elife 7:pii: e37093

    Article  Google Scholar 

  11. Giovannetti M, Mosse B (1980) Evaluation of techniques for measuring vesicular arbuscular mycorrhizal infection in roots. New Phytol 84(3):489–500

    Article  Google Scholar 

  12. Vierheilig H, Coughlan AP, Wyss U, Piché Y (1998) Ink and vinegar, a simple staining technique for arbuscular-mycorrhizal fungi. Appl Environ Microbiol 64:5004–5007

    CAS  PubMed  PubMed Central  Google Scholar 

  13. Fester T et al (2002) Stimulation of carotenoid metabolism in arbuscular mycorrhizal roots. Planta 216:148–154

    Article  CAS  Google Scholar 

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Acknowledgments

This work was supported by grants AGL2015-64990-C2-1-R and BIO2017-90877-REDT from the Ministry of Economy and Competitiveness (MINECO) and 201640I040 from CSIC.

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Correspondence to Juan A. López-Ráez .

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García, J.M., Pozo, M.J., López-Ráez, J.A. (2020). Histochemical and Molecular Quantification of Arbuscular Mycorrhiza Symbiosis. In: Rodríguez-Concepción, M., Welsch, R. (eds) Plant and Food Carotenoids. Methods in Molecular Biology, vol 2083. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9952-1_22

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  • DOI: https://doi.org/10.1007/978-1-4939-9952-1_22

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9951-4

  • Online ISBN: 978-1-4939-9952-1

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