Abstract
ATP synthase is an essential enzyme found in all known forms of life, generating the majority of cellular energy via a rotary catalytic mechanism. Here, we describe the in-depth methods for expression, purification, and functional assessment of E. coli ATP synthase.
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Sobti, M., Ishmukhametov, R., Stewart, A.G. (2020). ATP Synthase: Expression, Purification, and Function. In: Gerrard, J., Domigan, L. (eds) Protein Nanotechnology. Methods in Molecular Biology, vol 2073. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9869-2_5
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DOI: https://doi.org/10.1007/978-1-4939-9869-2_5
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