Abstract
Generating a transgenic or gene-modified mouse requires the introduction of exogenous reagents into an early-stage embryo. The mouse one-cell embryo or zygote possesses two pronuclei, representing the genetic contribution of the sperm and oocyte. Traditional transgenic mice are generated by injecting a DNA solution containing a purified transgene construct into the male pronucleus, generally the larger of the two pronuclei. Similarly, gene-editing reagents such as ZFNs, TALENs, and CRISPR RNAs are introduced into zygotes in the same manner, making this technique applicable to a wide variety of projects. This chapter presents the procedures for pronuclear microinjection.
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References
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Larson, M.A. (2020). Pronuclear Microinjection of One-Cell Embryos. In: Larson, M. (eds) Transgenic Mouse. Methods in Molecular Biology, vol 2066. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9837-1_2
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DOI: https://doi.org/10.1007/978-1-4939-9837-1_2
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Publisher Name: Humana, New York, NY
Print ISBN: 978-1-4939-9836-4
Online ISBN: 978-1-4939-9837-1
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