Abstract
Non-targeted metabolic analysis of single cells by mass spectrometry (MS) is important for understanding individual cell functions and characterizing cell-to-cell heterogeneity. However, identifying biomolecules in single cells presents significant challenges due to the low picoliter volume samples and the structural diversity of metabolites. Capillary microsampling electrospray ionization (ESI) MS with ion mobility separation (IMS) enables the analysis of single cells under ambient conditions with minimum sample pretreatment and improved specificity. Here, we describe a protocol for the analysis of the metabolic makeup, and the identification of ions produced from single cells by capillary microsampling ESI-IMS-MS.
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Acknowledgements
The authors acknowledge the financial support from the U.S. National Science Foundation under Grant No. CHE-1152302.
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Zhang, L., Allworth, L.L., Vertes, A. (2020). Identification of Metabolites in Single Cells by Ion Mobility Separation and Mass Spectrometry. In: Shrestha, B. (eds) Single Cell Metabolism. Methods in Molecular Biology, vol 2064. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9831-9_2
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DOI: https://doi.org/10.1007/978-1-4939-9831-9_2
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