Abstract
Regulation of the metabolic homeostasis is a prerequisite for all the animals for their growth and development. Thus, every organism developed strategies to maintain a balance between energy intake and expenditure. The imbalance in metabolism leads to a number of diseases such as obesity, diabetes, cardiovascular diseases and cancer. Besides the existence of structural variation between the organisms, the metabolic pathways are evolutionary conserved. Thus, the study of the metabolic pathways in a model system will help us to understand the role of metabolism in the onset of a disease. Drosophila melanogaster is evolving as an important model to study various metabolic disorders of humanbeing. This organism possesses physiological and metabolic similarity with mammals. Thus, various metabolic components like glucose, trehalose, protein and lipid associated with the fat-sensitive pathways as well as glucose-induced disorder can be checked using the fly model. The current chapter briefly employs various methods to quantify the metabolites from the larval as well as adult Drosophila tissues.
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Acknowledgements
SM is thankful to MHRD for financial support. MM lab is supported by Grant No. BT/PR21857/NNT/28/1238/2017, EMR/2017/003054, Odisha DBT 3325/ST(BIO)-02/2017.
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Mukherjee, S., Mishra, M. (2020). Biochemical Estimation to Detect the Metabolic Pathways of Drosophila. In: Mishra, M. (eds) Fundamental Approaches to Screen Abnormalities in Drosophila. Springer Protocols Handbooks. Springer, New York, NY. https://doi.org/10.1007/978-1-4939-9756-5_12
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DOI: https://doi.org/10.1007/978-1-4939-9756-5_12
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