Abstract
In recent years cancer immunotherapy, especially the cell-based immunotherapy, has reached several milestones and achieved a lot of cancer remission in the clinics. Obtaining a more potent and effective cytotoxic T lymphocytes (CTLs) for cancer immunotherapy is always the ultimate goal for the researchers. However, the difficulty in harvesting a large number of tumor antigen-specific CTLs from the tumor patient is still a major obstacle we need to overcome. In our previous studies, it is shown that pluripotent stem cell-derived CTL—especially the genetically engineered antigen-specific CTLs—may serve as a good source of unlimited number of highly reactive and antigen-specific CTLs. Here we present a two-step method for the generation of antigen-specific T lymphocytes from iPS cells by in vitro priming and in vivo maturation.
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Acknowledgments
This project was funded, in part, under the grants from National Institute of Health Grant R01AI121180, R21AI109239, and R01CA221867 and the American Diabetes Association (1-16-IBS-281) to J.S.
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Chen, X., Lei, F., Wang, L., Xiong, X., Song, J. (2019). In Vitro Differentiation of T Cells from Murine Pluripotent Stem Cells. In: Kaneko, S. (eds) In Vitro Differentiation of T-Cells. Methods in Molecular Biology, vol 2048. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9728-2_14
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DOI: https://doi.org/10.1007/978-1-4939-9728-2_14
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