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Biophysical Techniques to Analyze Elastic Tissue Extracellular Matrix Proteins Interacting with ADAMTS Proteins

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ADAMTS Proteases

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2043))

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Abstract

Multidomain matrix-associated zinc extracellular proteases ADAMTS and ADAMTS-like proteins have important biological activities in cells and tissues. Beyond their traditional role in procollagen and von Willebrand factor processing and proteoglycan cleavage, ADAMTS/ADAMTSL likely participate in or at least have some role in ECM assembly as some of these proteins bind ECM proteins including fibrillins, fibronectin, and LTBPs. In this chapter, we present four biophysical techniques largely used for the characterization, multimerization, and interaction of proteins: surface plasmon resonance spectroscopy, dynamic light scattering, atomic force microscopy, and circular dichroism spectroscopy.

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Author information

Authors and Affiliations

  1. Faculty of Dentistry, McGill University, Montreal, QC, Canada

    Valentin Nelea & Dieter P. Reinhardt

  2. Faculty of Medicine, Department of Anatomy and Cell Biology, McGill University, Montreal, QC, Canada

    Valentin Nelea & Dieter P. Reinhardt

Authors
  1. Valentin Nelea
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  2. Dieter P. Reinhardt
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Corresponding author

Correspondence to Dieter P. Reinhardt .

Editor information

Editors and Affiliations

  1. Department of Biomedical Engineering-ND20, Cleveland Clinic Lerner Research Institute, Cleveland, OH, USA

    Suneel S. Apte

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Nelea, V., Reinhardt, D.P. (2020). Biophysical Techniques to Analyze Elastic Tissue Extracellular Matrix Proteins Interacting with ADAMTS Proteins. In: Apte, S. (eds) ADAMTS Proteases. Methods in Molecular Biology, vol 2043. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9698-8_18

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  • DOI: https://doi.org/10.1007/978-1-4939-9698-8_18

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9697-1

  • Online ISBN: 978-1-4939-9698-8

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