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A Coinfection Model to Evaluate Chlamydia Inc Protein Interactions

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Chlamydia trachomatis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2042))

Abstract

Chlamydia trachomatis resides and replicates within a membranous vacuole, termed the inclusion. A group of Type III secreted effector proteins, the inclusion membrane proteins (Inc), are embedded within the inclusion membrane and facilitate the interaction of the inclusion with host cell organelles. These interactions are vital for bacterial replication and allow for the acquisition of essential nutrients from the host cell. However, it is not known if Inc proteins function independently or require interactions with other Inc proteins to function. This chapter describes a system to test the homotypic/heterotypic interactions of Inc proteins through the coinfection of Chlamydia strains expressing differently tagged inclusion membrane proteins. Our approach takes advantage of the natural homotypic fusion of inclusions and allows for the study of Inc protein interactions when they are embedded within the inclusion membrane.

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Acknowledgments

This work was supported by NIAID grant R01AI101441 to ID. We thank Rebecca Stanhope, Maria Cortina, and Ralph Bishop for reviewing the manuscript.

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Authors and Affiliations

  1. Department of Microbiology, Immunology, and Cancer Biology, University of Virginia, Charlottesville, VA, USA

    Rachel Ende & Isabelle Derré

Authors
  1. Rachel Ende
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  2. Isabelle Derré
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Corresponding author

Correspondence to Isabelle Derré .

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Editors and Affiliations

  1. Department of Microbiology and Immunology, Vet School, Cornell University, Ithaca, NY, USA

    Amanda Claire Brown

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Ende, R., Derré, I. (2019). A Coinfection Model to Evaluate Chlamydia Inc Protein Interactions. In: Brown, A. (eds) Chlamydia trachomatis. Methods in Molecular Biology, vol 2042. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9694-0_14

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  • DOI: https://doi.org/10.1007/978-1-4939-9694-0_14

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9693-3

  • Online ISBN: 978-1-4939-9694-0

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