Abstract
NMR spectroscopy is one of the major analytical techniques used in the metabolomics studies of food. There are many applications of metabolomics on food-related topics and on the food itself. Here, we describe protocols for performing NMR-based metabolomics of foods ranging from simple beverages to solid foods and semisolid foods. Beverages can be analyzed either directly or after sample preprocessing to remove interfering macromolecules, muscle-based foods can be analyzed after extraction, and semisolid foods can be analyzed directly using high-resolution magic-angle spinning (HR-MAS) NMR. Finally, we discuss metabolomic data analysis as well as different procedures and strategies for targeted and untargeted approaches.
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Acknowledgements
HCB acknowledges the Independent Research Fund Denmark for financial support through her Eliteforsk 2017 grant (# 6161-00016B).
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Sundekilde, U.K., Eggers, N., Bertram, H.C. (2019). NMR-Based Metabolomics of Food. In: Gowda, G., Raftery, D. (eds) NMR-Based Metabolomics. Methods in Molecular Biology, vol 2037. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9690-2_18
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DOI: https://doi.org/10.1007/978-1-4939-9690-2_18
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