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In Vitro Cytogenetic Assays: Chromosomal Aberrations and Micronucleus Tests

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Genotoxicity Assessment

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2031))

Abstract

Chromosome damage is a very important indicator of genetic damage relevant to environmental and clinical studies. Detailed descriptions of the protocols used for detection of chromosomal aberrations induced by genotoxic agents in vitro both in the presence or absence of rat liver-derived metabolizing systems are given in this chapter. Structural chromosomal aberrations that can be observed and quantified at metaphases are described here. For the detection of chromosomal damage (fragments or whole chromosome) in interphase, the micronucleus test can be used, and a description of this test is also presented. Criteria for determining a positive result using appropriate statistical methods are described.

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Authors and Affiliations

  1. Dipartimento di Scienze Ecologiche e Biologiche, Università degli Studi della Tuscia, Largo dell’Università, Viterbo, Italy

    Pasquale Mosesso

  2. Research Toxicology Centre S.p.A., Pomezia (Roma), Italy

    Serena Cinelli

Authors
  1. Pasquale Mosesso
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  2. Serena Cinelli
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Corresponding author

Correspondence to Pasquale Mosesso .

Editor information

Editors and Affiliations

  1. CSIR—Indian Institute of Toxicology Research, Lucknow, Uttar Pradesh, India

    Alok Dhawan

  2. New Delhi, Delhi, India

    Mahima Bajpayee

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Mosesso, P., Cinelli, S. (2019). In Vitro Cytogenetic Assays: Chromosomal Aberrations and Micronucleus Tests. In: Dhawan, A., Bajpayee, M. (eds) Genotoxicity Assessment. Methods in Molecular Biology, vol 2031. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9646-9_4

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  • DOI: https://doi.org/10.1007/978-1-4939-9646-9_4

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9645-2

  • Online ISBN: 978-1-4939-9646-9

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