Abstract
The Tradescantia micronucleus assay has been used since 50 years for the detection of genotoxins (including carcinogens) in the environment. A large database concerning the effects of individual chemicals and complex environmental mixtures (soil, air and waters) has accumulated. In contrast to other mutagenicity test systems, the effects of low concentrations of heavy metals, radionuclides, certain herbicides, pesticides and gaseous mutagens can be detected and it is also possible to conduct in situ biomonitoring studies with plant. The test system has been validated and standardized protocols have been developed for laboratory experiments and for field studies which are described in this chapter.
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Acknowledgments
We thank P. Breit and E. Gottmann for help in the preparation of the photographic images.
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Mišík, M., Pichler, C., Rainer, B., Nersesyan, A., Mišíková, K., Knasmueller, S. (2019). Micronucleus Assay with Tetrad Cells of Tradescantia. In: Dhawan, A., Bajpayee, M. (eds) Genotoxicity Assessment. Methods in Molecular Biology, vol 2031. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9646-9_18
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DOI: https://doi.org/10.1007/978-1-4939-9646-9_18
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