Abstract
Coexpression of a plant NB-LRR-type resistance (R) gene and corresponding viral avirulent (Avr) gene introduced in Nicotiana benthamiana using Agrobacterium tumefaciens confers hypersensitive response (HR). Such Agrobacterium-mediated transient gene expression methods have contributed to the identification of new plant R genes and facilitated the analysis of their functions. Here we describe a model method, by which several tobamovirus R genes from Solanaceous plants have been successfully identified and characterized molecularly.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Similar content being viewed by others
References
Jones JD, Dangl JL (2006) The plant immune system. Nature 444:323–329
Cook DE, Mesarich CH, Thomma BP (2015) Understanding plant immunity as a surveillance system to detect invasion. Annu Rev Phytopathol 53:541–563
Heath MC (2000) Hypersensitive response-related death. Plant Mol Biol 44:321–334
Collier SM, Moffett P (2009) NB-LRRs work a “bait and switch” on pathogens. Trends Plant Sci 14:521–529
Whitham S, Dinesh-Kumar SP, Choi D, Hehl R, Corr C, Baker B (1994) The product of the tobacco mosaic virus resistance gene N: similarity to toll and the interleukin-1 receptor. Cell 78:1101–1115
de Ronde D, Butterbach P, Kormelink R (2014) Dominant resistance against plant viruses. Front Plant Sci 5:307
Takken FL, Tameling WI (2009) To nibble at plant resistance proteins. Science 324:744–746
Meyers BC, Kozik A, Griego A, Kuang H, Michelmore RW (2003) Genome-wide analysis of NBS-LRR-encoding genes in Arabidopsis. Plant Cell 15:809–834
Zhou T, Wang Y, Chen JQ, Araki H, Jing Z, Jiang K, Shen J, Tian D (2004) Genome-wide identification of NBS genes in japonica rice reveals significant expansion of divergent non-TIR NBS-LRR genes. Mol Gen Genomics 271:402–415
Kohler A, Rinaldi C, Duplessis S, Baucher M, Geelen D, Duchaussoy F, Meyers BC, Boerjan W, Martin F (2008) Genome-wide identification of NBS resistance genes in Populus trichocarpa. Plant Mol Biol 66:619–636
Jupe F, Pritchard L, Etherington GJ, Mackenzie K, Cock PJ, Wright F, Sharma SK, Bolser D, Bryan GJ, Jones JD, Hein I (2012) Identification and localisation of the NB-LRR gene family within the potato genome. BMC Genomics 13:75
Whitham S, McCormick S, Baker B (1996) The N gene of tobacco confers resistance to tobacco mosaic virus in transgenic tomato. Proc Natl Acad Sci U S A 93:8776–8781
Takahashi H, Miller J, Nozaki Y, Takeda M, Shah J, Hase S, Ikegami M, Ehara Y, Dinesh-Kumar SP (2002) RCY1, an Arabidopsis thaliana RPP8/HRT family resistance gene, conferring resistance to cucumber mosaic virus requires salicylic acid, ethylene and a novel signal transduction mechanism. Plant J 32:655–667
Sekine KT, Kawakami S, Hase S, Kubota M, Ichinose Y, Shah J, Kang HG, Klessig DF, Takahashi H (2008) High level expression of a virus resistance gene, RCY1, confers extreme resistance to Cucumber mosaic virus in Arabidopsis thaliana. Mol Plant-Microbe Interact 21:1398–1407
Bendahmane A, Querci M, Kanyuka K, Baulcombe DC (2000) Agrobacterium transient expression system as a tool for the isolation of disease resistance genes: application to the Rx2 locus in potato. Plant J 21:73–81
Mestre P, Baulcombe DC (2006) Elicitor-mediated oligomerization of the tobacco N disease resistance protein. Plant Cell 18:491–501
Rairdan GJ, Moffett P (2006) Distinct domains in the ARC region of the potato resistance protein Rx mediate LRR binding and inhibition of activation. Plant Cell 18:2082–2093
Sacco MA, Mansoor S, Moffett P (2007) A RanGAP protein physically interacts with the NB-LRR protein Rx, and is required for Rx-mediated viral resistance. Plant J 52:82–93
Tomita R, Sekine KT, Mizumoto H, Sakamoto M, Murai J, Kiba A, Hikichi Y, Suzuki K, Kobayashi K (2011) Genetic basis for the hierarchical interaction between Tobamovirus spp. and L resistance gene alleles from different pepper species. Mol Plant-Microbe Interact 24:108–117
Sekine KT, Tomita R, Takeuchi S, Atsumi G, Saitoh H, Mizumoto H, Kiba A, Yamaoka N, Nishiguchi M, Hikichi Y, Kobayashi K (2012) Functional differentiation in the Leucine-rich repeat domains of closely related plant virus-resistance that recognize common Avr proteins. Mol Plant-Microbe Interact 25:1219–1229
Hamel LP, Sekine KT, Wallon T, Sugiwaka Y, Kobayashi K, Moffett P (2016) The chloroplastic protein THF1 interacts with the coiled-coil domain of the disease resistance protein N′ and regulates light-dependent cell death. Plant Physiol 171:658–674
Hellens RP, Edwards EA, Leyland NR, Bean S, Mullineaux PM (2000) pGreen: a versatile and flexible binary Ti vector for Agrobacterium-mediated plant transformation. Plant Mol Biol 42:819–832
Takahashi H, Shoji H, Ando S, Kanayama Y, Kusano T, Takeshita M, Suzuki M, Masuta C (2012) RCY1-mediated resistance to Cucumber mosaic virus is regulated by LRR domain-mediated interaction with CMV(Y) following degradation of RCY1. Mol Plant-Microbe Interact 25:1171–1185
Acknowledgments
This work was supported in part by JSPS KAKENHI grant numbers 24780043 and 17K15230.
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2019 Springer Science+Business Media, LLC, part of Springer Nature
About this protocol
Cite this protocol
Tomita, R., Sekine, KT., Tateda, C., Kobayashi, K. (2019). Identification and Functional Analysis of NB-LRR-Type Virus Resistance Genes: Overview and Functional Analysis of Candidate Genes. In: Kobayashi, K., Nishiguchi, M. (eds) Antiviral Resistance in Plants. Methods in Molecular Biology, vol 2028. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9635-3_1
Download citation
DOI: https://doi.org/10.1007/978-1-4939-9635-3_1
Published:
Publisher Name: Humana, New York, NY
Print ISBN: 978-1-4939-9634-6
Online ISBN: 978-1-4939-9635-3
eBook Packages: Springer Protocols