Abstract
The biology of fertility, early development, and pregnancy is variable across mammalian species. In addition, while the physiology and pathophysiology of human diseases can be investigated in other animal models (principally, rodents), differences between human and lower mammals often present limitations in the applicability of physiological processes from rodent models to human biology. Since 1984, when the first live birth from rhesus monkey in vitro fertilization and embryo transfer was reported (Bavister et al., Proc Natl Acad Sci 81:2218–2222, 1984), there has been progress in the implementation of assisted reproductive technologies with several nonhuman primate (NHP) species that play important roles in biomedical research. In recent years, the significance of this progress has been amplified by the development of genomic editing approaches for facile genetic manipulation of the embryo, including methods now applied to NHPs (Liu et al., Cell Stem Cell 14:323–328, 2014; Niu et al., Cell 156:836–843, 2014). In this review, we summarize current protocols and practices for the common marmoset. It is our intention to provide current state-of-the-art protocols for gamete procurement and in vitro fertilization techniques, so that laboratories wishing to implement experimental embryology in marmoset models will have a basic set of tools with which to initiate such studies.
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Acknowledgments
T.G.G. and J.K.S. would like to thank the veterinary and animal care staff at the Wisconsin National Primate Research Center, particularly Michele Schotzko and Dr. Nancy Schultz-Darken of the Scientific Protocol Implementation Unit, Megan Sosa of the Animal Services Unit, and Drs. Casey Fitz and Kevin Brunner of the Veterinary Services Unit, for assistance in synchronizing donors and collection of gametes to perform marmoset in vitro experiments to generate embryo images. Studies at the University of Wisconsin-Madison were supported by NIH grants R24OD019803 to T.G.G. and P51OD011106 to the WNPRC.
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Schmidt, J.K., Golos, T.G. (2019). In Vitro Culture of Embryos from the Common Marmoset (Callithrix jacchus). In: Herrick, J. (eds) Comparative Embryo Culture. Methods in Molecular Biology, vol 2006. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9566-0_21
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DOI: https://doi.org/10.1007/978-1-4939-9566-0_21
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