Abstract
Tumor cells grown as spheroids more closely resemble in vivo solid tumors and present physical and physiological barriers to drug action that conventional monolayer cell cultures do not. The physiological relevance of the model can be further increased by incorporating fluid flow and multiple cell types. The protocols described in this chapter use simple and easily available equipment to reproducibly generate spheroid models that can be used for the in vitro testing of a variety of preparations.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Similar content being viewed by others
References
Riedl A, Schlederer M, Pudelko K, Stadler M, Walter S, Unterleuthner D et al (2017) Comparison of cancer cells in 2D vs 3D culture reveals differences in AKT-mTOR-S6K signaling and drug responses. J Cell Sci 130(1):203–218
Xu X, Farach-Carson MC, Jia X (2014) Three-dimensional in vitro tumor models for cancer research and drug evaluation. Biotechnol Adv 32(7):1256–1268
Atefi E, Lemmo S, Fyffe D, Luker GD, Tavana H (2014) High throughput, polymeric aqueous two-phase printing of tumor spheroids. Adv Funct Mater 24(41):6509–6515
Ivanov DP, Parker TL, Walker DA, Alexander C, Ashford MB, Gellert PR et al (2014) Multiplexing spheroid volume, resazurin and acid phosphatase viability assays for high-throughput screening of tumour spheroids and stem cell neurospheres. PLoS One 9(8):e103817
Monjaret F, Fernandes M, Duchemin-Pelletier E, Argento A, Degot S, Young J (2016) Fully automated one-step production of functional 3D tumor spheroids for high-content screening. J Lab Autom 21(2):268–280
Sabhachandani P, Motwani V, Cohen N, Sarkar S, Torchilin V, Konry T (2016) Generation and functional assessment of 3D multicellular spheroids in droplet based microfluidics platform. Lab Chip 16(3):497–505
Lao Z, Kelly CJ, Yang XY, Jenkins WT, Toorens E, Ganguly T et al (2015) Improved methods to generate spheroid cultures from tumor cells, tumor cells & fibroblasts or tumor-fragments: microenvironment, microvesicles and MiRNA. PLoS One 10(7):e0133895
Solomon MA, Lemera J, D’Souza GG (2016) Development of an in vitro tumor spheroid culture model amenable to high-throughput testing of potential anticancer nanotherapeutics. J Liposome Res 26(3):246–260
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2019 Springer Science+Business Media, LLC, part of Springer Nature
About this protocol
Cite this protocol
Ganpule, A., Gui, Z., Almuteri, M.A., D’Souza, G.G.M. (2019). In Vitro Testing of Anticancer Nanotherapeutics Using Tumor Spheroids. In: Weissig, V., Elbayoumi, T. (eds) Pharmaceutical Nanotechnology. Methods in Molecular Biology, vol 2000. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9516-5_24
Download citation
DOI: https://doi.org/10.1007/978-1-4939-9516-5_24
Published:
Publisher Name: Humana, New York, NY
Print ISBN: 978-1-4939-9515-8
Online ISBN: 978-1-4939-9516-5
eBook Packages: Springer Protocols