Abstract
Capillary zone electrophoresis (CZE) complemented with Taylor Dispersion Analysis-CE (TDA-CE) was developed to physicochemically characterize phthalocyanine-capped core/shell/shell quantum dots (QDs) at various pH and ionic strengths. An LED-induced fluorescence detector was used to specifically detect the QDs. The electropherograms and taylorgrams allowed calculating the phthalocyanine-QDs (Pc-QDs) ζ-potential and size, respectively, and determining the experimental conditions for colloidal stability. This methodology allowed evidencing either a colloidal stability or an aggregation state according to the background electrolytes nature. The calculated ζ-potential values of Pc-QDs decreased when ionic strength increased, being well correlated with the aggregation of the nanoconjugates at elevated salt concentrations. For the same reason, the hydrodynamic diameter of Pc-QDs increased with increasing background electrolyte ionic strength. The use of electrokinetic methodologies has provided insights into the colloidal stability of the photosensitizer-functionalized QDs in physiologically relevant solutions and, thereby, its usefulness for improving their design and applications for photodynamic therapy.
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Acknowledgments
This work was partially supported by the Department of Science and Technology (DST) and National Research Foundation (NRF) of South Africa, through the DST/NRF South African Research Chairs Initiative for Professor of Medicinal Chemistry and Nanotechnology (UID = 62620) and Rhodes University and by DST/Mintek Nanotechnology Innovation Centre (NIC). GRG is grateful to the Mexican National Council for Science and Technology (CONACYT). The authors acknowledge financial support from PROTEA Project 33885ZJ (France–South Africa).
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Ramírez-García, G., d’Orlyé, F., Nyokong, T., Bedioui, F., Varenne, A. (2019). Physicochemical Characterization of Phthalocyanine-Functionalized Quantum Dots by Capillary Electrophoresis Coupled to a LED Fluorescence Detector. In: Weissig, V., Elbayoumi, T. (eds) Pharmaceutical Nanotechnology. Methods in Molecular Biology, vol 2000. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9516-5_23
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