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Raman Spectroscopy in Nonwoody Plants

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Plant Cell Morphogenesis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1992))

Abstract

Confocal Raman spectroscopy (RS) enables obtaining molecular information from the nondestructive analysis of plant material in situ. It can thereby be a useful method to investigate spatial distribution and heterogeneity of cell-wall polymers. The authors’ intention is to present some examples of RS application and its capabilities for investigations of nonwoody plants. In this context, we present protocols for qualitative analysis of main polymers of plant wall and application of RS in a semiquantitative study of the arrangement of selected polymers in the wall in its native state.

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Acknowledgments

This work was financially supported by the National Science Centre, Poland (ERA-CAPS CALL 2016; project No. 2017/24/Z/NZ3/00548 and 2017/26/D/ST8/01117). We thank Dorota Kwiatkowska and Roman Wrzalik for discussion and comments.

Author information

Authors and Affiliations

  1. Department of Biophysics and Morphogenesis of Plants, University of Silesia in Katowice, Katowice, Poland

    Dorota Borowska-Wykręt

  2. Institute of Material Science, University of Silesia in Katowice, Chorzów, Poland

    Mateusz Dulski

  3. Silesian Center for Education and Interdisciplinary Research, Chorzów, Poland

    Mateusz Dulski

Authors
  1. Dorota Borowska-Wykręt
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  2. Mateusz Dulski
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Corresponding author

Correspondence to Dorota Borowska-Wykręt .

Editor information

Editors and Affiliations

  1. Department of Experimental Plant Biology, Charles University, Prague, Czech Republic

    Fatima Cvrčková

  2. Department of Experimental Plant Biology, Charles University, Prague, Czech Republic

    Viktor Žárský

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Borowska-Wykręt, D., Dulski, M. (2019). Raman Spectroscopy in Nonwoody Plants. In: Cvrčková, F., Žárský, V. (eds) Plant Cell Morphogenesis. Methods in Molecular Biology, vol 1992. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9469-4_6

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  • DOI: https://doi.org/10.1007/978-1-4939-9469-4_6

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9468-7

  • Online ISBN: 978-1-4939-9469-4

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