Abstract
Sampling and handling of soils and rhizosphere soil are very critical steps for obtaining representative microbial cultures and genomic material from these environments. Attention to position in the landscape of a sampling site, previous management of the site, time of year, and depth of sampling is important to assure representative samples. Detailed protocols are provided to assist environmental microbiologists and molecular biologists in proper sampling and handling of soils that serve as the source of cultures and DNA for subsequent use in important experiments carried out in the laboratory.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Similar content being viewed by others
References
Cole JR, Myrold DD, Nakatsu CH et al (2010) Development of soil metadata standards for international DNA sequence databases. 19th World Congress of Soil Science, Soil Solutions for a Changing World, 1-6 August, Brisbane, Australia, p 5–8
Lorenz N, Dick RP (2011) Sampling and pretreatment of soil before enzyme analysis. In: Dick RP (ed) Methods of soil enzymology, SSSA Book Series no 9. Soil Science Society of America, Madison, WI, p 85
Schutter M, Sandeno J, Dick RP (2001) Seasonal, soil type, and alternative managementinfluences on microbial communities of vegetable cropping systems. Biol Fertil Soils 34:397–410
Fang M, Kremer RJ, Motavalli PP et al (2005) Bacterial diversity in rhizospheres of non-transgenic and transgenic corn. Appl Environ Microbiol 71:4132–4413
Schoeneberger PJ, Wysocki DA, Benham EC (2012) Field book for describing and sampling soils. Natural Resources Conservation Service, National Soil Survey Center, Lincoln, NE, p 334
Veum KS, Sudduth KA, Kremer RJ et al (2017) Sensor data fusion for soil health assessment. Geoderma 305:53–61
Franzluebbers AJ, Haney RL (2018) Evaluation of soil processing conditions on meneralizable C and N across a textural gradient. Soil Sci Soc Am J 82:1299–1305
Ndaw SM, Gama-Rodrigues AC, Gama-Rodrigues EF et al (2009) Relationships between bacterial diversity, microbial biomass, and litter quality in soils under different plant covers in northern Rio de Janeiro State, Brazil. Can J Microbiol 55:1089–1095
Lee YB, Lorenz N, Dick LK et al (2007) Cold storage and pretreatment incubation effects on soil microbial properties. Soil Sci Soc Am J 71:354–361
Kremer RJ (2013) Interactions between plants and microorganisms. Allelopath J 31:51–70
Brady NC, Weil RR (2008) The nature and properties of soils. Pearson-Prentice Hall, Upper Saddle River, NJ
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2019 Springer Science+Business Media, LLC, part of Springer Nature
About this protocol
Cite this protocol
Kremer, R.J. (2019). Sampling and Handling of Soil to Identify Microorganisms with Impacts on Plant Growth. In: Gassmann, W. (eds) Plant Innate Immunity. Methods in Molecular Biology, vol 1991. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9458-8_21
Download citation
DOI: https://doi.org/10.1007/978-1-4939-9458-8_21
Published:
Publisher Name: Humana, New York, NY
Print ISBN: 978-1-4939-9457-1
Online ISBN: 978-1-4939-9458-8
eBook Packages: Springer Protocols