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Production of Recombinant Trypanosoma cruzi Antigens in Leishmania tarentolae

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T. cruzi Infection

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1955))

Abstract

Trypanosomatids are unicellular organisms that colonize a wide diversity of environments and hosts. For instance, Trypanosoma cruzi is a human pathogen responsible for Chagas diseases, while Leishmania tarentolae infects amphibians and became a biotechnological tool suitable for recombinant protein expression. T. cruzi antigens are needed for the development of improved epitope-based methods for diagnosis and treatment of Chagas disease. Molecular cloning for the production of recombinant proteins offers the possibility to obtain T. cruzi antigens at high yield and purity. L. tarentolae appears as the ideal expression host to obtain recombinant T. cruzi antigens with a structure and posttranslational modifications typical of trypanosomatids. In this chapter, we present a protocol for the analytical to mid-scale production of recombinant T. cruzi antigens, using L. tarentolae as expression host (LEXSY® inducible system).

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Acknowledgments

This work was supported by grants to M.T.T.I. and M.P. (PIP 2015-0937 and PICT 2016-1028). M.A.C. acknowledges the financial support of FOCEM (MERCOSUR Structural Convergence Fund, [COF 03/11]).

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Authors and Affiliations

  1. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular, “Dr. Héctor Torres” (INGEBI-CONICET), Buenos Aires, Argentina

    María José Ferrer, Diana Patricia Wehrendt, María Teresa Tellez-Iñón & Mariana Potenza

  2. Group Redox Biology of Trypanosomes, Institut Pasteur de Montevideo, Montevideo, Uruguay

    Mariana Bonilla & Marcelo Alberto Comini

Authors
  1. María José Ferrer
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  2. Diana Patricia Wehrendt
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  3. Mariana Bonilla
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  4. Marcelo Alberto Comini
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  5. María Teresa Tellez-Iñón
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  6. Mariana Potenza
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Corresponding author

Correspondence to Mariana Potenza .

Editor information

Editors and Affiliations

  1. INGEBI-CONICET, Buenos Aires, Argentina

    Karina Andrea Gómez

  2. UNSAM-CONICET, IIB-INTECH, San Martin, Buenos Aires, Argentina

    Carlos Andrés Buscaglia

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Ferrer, M.J., Wehrendt, D.P., Bonilla, M., Comini, M.A., Tellez-Iñón, M.T., Potenza, M. (2019). Production of Recombinant Trypanosoma cruzi Antigens in Leishmania tarentolae. In: Gómez, K., Buscaglia, C. (eds) T. cruzi Infection. Methods in Molecular Biology, vol 1955. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-9148-8_8

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  • DOI: https://doi.org/10.1007/978-1-4939-9148-8_8

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-9147-1

  • Online ISBN: 978-1-4939-9148-8

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