Abstract
RNA-seq is a powerful method to study in detail transcriptome changes in defined conditions, providing enormous amount of information on RNA stability and gene regulation. In this chapter, we describe a directional and a nondirectional library preparation protocol for RNA-seq in Trypanosoma cruzi, as well as a pipeline for bioinformatic analysis, which includes read trimming, alignment to a reference genome, and differential expression.
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Supplementary Material 1
Transcripts abundance quantification using Erange (PDF 228 kb)
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DESeq pipeline (DOCX 14 kb)
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Greif, G., Berná, L., Díaz-Viraqué, F., Robello, C. (2019). Transcriptome Studies in Trypanosoma cruzi Using RNA-seq. In: Gómez, K., Buscaglia, C. (eds) T. cruzi Infection. Methods in Molecular Biology, vol 1955. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-9148-8_3
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DOI: https://doi.org/10.1007/978-1-4939-9148-8_3
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