Abstract
Physiological responses, such as phototropism and carotenogenesis, are usually exhibited through a specific cascade composed of several gene functions. The number of such gene functions can be determined by means of complementation analysis. For this purpose, a procedure is needed to produce heterokaryons easily and with a high success rate. Here, we present a method of grafting sporangiophores from different mutants to obtain heterokaryotic regenerates at the graft union, based on the large size of the sporangiophore and its remarkable regeneration capability.
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Miyazaki, A., Ootaki, T. (2019). Grafting as a Tool for Study of Phototropism in Phycomyces. In: Yamamoto, K. (eds) Phototropism. Methods in Molecular Biology, vol 1924. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-9015-3_16
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DOI: https://doi.org/10.1007/978-1-4939-9015-3_16
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Publisher Name: Humana Press, New York, NY
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