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TILLING in Barley

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Barley

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1900))

Abstract

TILLING (Targeting Induced Local Lesions IN Genomes), a popular reverse genetics approach in barley research, combines plant mutagenesis with efficient mutation detection for studying biological function of a specific gene. The high mutation frequency within a TILLING population principally enables the identification of induced variations in (almost) all genes of a given species (more precisely a given genotype of a species) of interest, which can be tested for their functional impact on morphological and/or physiological characteristics of the plant. Several TILLING populations induced by chemical mutagenesis were established for barley (Talame et al., Plant Biotechnol J 6:477–485, 2008; Gottwald et al., BMC Res Notes 2:258, 2009; Caldwell et al. Plant J 40:143–150, 2004) and showed the possibility for adapting protocols to develop further populations. This chapter describes a chemical mutagenesis protocol for barley seeds and two independent procedures for efficient single nucleotide polymorphism (SNP) detection in a large number of mutagenized plants either by slab-gel- or capillary gel-based electrophoreses on the LI-COR 4300 DNA Analyzer and the AdvanCE FS96 instruments, respectively.

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Acknowledgements

The work was supported by the European Regional Development Fund through the Innovative Economy for Poland 2007–2013, project WND-POIG.01.03.01-00-101/08 POLAPGEN-BD, task 22.

Author information

Authors and Affiliations

  1. Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Seeland, Germany

    Matthias Jost & Nils Stein

  2. Faculty of Biology and Environmental Protection, Department of Genetics, University of Silesia, Katowice, Poland

    Miriam Szurman-Zubrzycka, Katarzyna Gajek & Iwona Szarejko

Authors
  1. Matthias Jost
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  2. Miriam Szurman-Zubrzycka
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  3. Katarzyna Gajek
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  4. Iwona Szarejko
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  5. Nils Stein
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Corresponding author

Correspondence to Nils Stein .

Editor information

Editors and Affiliations

  1. Crop Transformation Group, Department of Crop Genetics, John Innes Centre, Norwich, UK

    Wendy A. Harwood

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Jost, M., Szurman-Zubrzycka, M., Gajek, K., Szarejko, I., Stein, N. (2019). TILLING in Barley. In: Harwood, W. (eds) Barley. Methods in Molecular Biology, vol 1900. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8944-7_6

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  • DOI: https://doi.org/10.1007/978-1-4939-8944-7_6

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8942-3

  • Online ISBN: 978-1-4939-8944-7

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