Abstract
Cutting frozen sections is a technique that allows for the rapid diagnosis of unfixed frozen tissue. A thin slice of tissue is cut from a frozen biospecimen and then stained typically with hematoxylin and eosin. The applications of this technique range from clinical diagnosis to quality assurance in the procurement or the use of biobanking specimens. It is important that the quality of the frozen section is of the highest possible caliber in order to optimize the pathologist’s interpretation, whether the result is used for surgical management or to validate the tissue being collected for research. Herein, we describe a step-by-step method to cut frozen sections.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Similar content being viewed by others
References
Wilson LB (1905) A method for the rapid preparation of fresh tissues for the microscope. JAMA XLV(23):1737. https://doi.org/10.1001/jama.1905.52510230037003c
Dahlin DC (1980). Seventy-five years’ experience with frozen sections at the Mayo Clinic. In: Mayo Clinic proceedings, Mayo Clinic, Rochester, November 1980. Vol 55. Elsevier, pp 721–723
Gal AA, Cagle PT (2005) The 100-year anniversary of the description of the frozen section procedure. JAMA 294(24):3135–3137. https://doi.org/10.1001/jama.294.24.3135
Marchevsky AM, Balzer B, Abdul-Karim FW (2014) Intraoperative consultation: a volume in the series: foundations in diagnostic pathology. Elsevier Saunders, Philadelphia
Peters SR (2010) A practical guide to frozen section technique. Springer, New York
Acknowledgments
We would like to thank Tim Wang for his frozen section expertise. Photographs taken by Jackie Bauzon Arcega.
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2019 Springer Science+Business Media, LLC, part of Springer Nature
About this protocol
Cite this protocol
Arcega, R.S., Woo, J.S., Xu, H. (2019). Performing and Cutting Frozen Sections. In: Yong, W. (eds) Biobanking. Methods in Molecular Biology, vol 1897. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8935-5_24
Download citation
DOI: https://doi.org/10.1007/978-1-4939-8935-5_24
Published:
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-8933-1
Online ISBN: 978-1-4939-8935-5
eBook Packages: Springer Protocols