Abstract
Chromatin immunoprecipitation (ChIP) is a powerful and sensitive technique that is widely used to study DNA-protein interactions. It enables an unbiased genome-wide analysis of transcriptional changes during several biological processes including cellular differentiation. Here, we describe a step-by-step protocol to identify histone modifications, transcription factor, and co-factor binding to chromatin in skeletal myoblasts. We discuss critical steps during cell harvesting, sonication, and immunoprecipitation and provide notes to evade common pitfalls.
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Work in the R.T. laboratory is supported by grants from the National Medical Research Council.
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Rao, V.K., Shankar, S.R., Taneja, R. (2019). Chromatin Immunoprecipitation in Skeletal Myoblasts. In: Rønning, S. (eds) Myogenesis. Methods in Molecular Biology, vol 1889. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8897-6_4
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DOI: https://doi.org/10.1007/978-1-4939-8897-6_4
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