Abstract
Coeliac disease (CD) is a T-cell mediated autoimmune disorder triggered by ingestion of cereal gluten found in wheat (gliadins and glutenins), barley (hordeins), and rye (secalins). As the only treatment for CD is a lifelong gluten-free diet, the measurement of gluten in raw ingredients and processed food products is critical to protecting people with CD or gluten intolerance. The most commonly employed method is the enzyme-linked immunosorbent assay (ELISA), but more recently mass spectrometry has been employed wherein the extracted gluten proteins are digested to peptides that are then directly measured. To achieve the goal of accurate gluten quantitation, gluten must be efficiently extracted from the ingredient or food matrix and then digested to yield the peptides that are monitored by LC-MS. In this chapter, a rapid, simple, and reproducible protocol for extraction and digestion of gluten proteins is described.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Similar content being viewed by others
References
Guandalini S, Assiri A (2014) Celiac disease: a review. JAMA Pediatr 168(3):272–278
Colgrave ML, Byrne K, Blundell M et al (2016) Comparing multiple reaction monitoring and sequential window acquisition of all theoretical mass spectra for the relative quantification of barley gluten in selectively bred barley lines. Anal Chem 88(18):9127–9135
Stamnaes J, Sollid LM (2015) Celiac disease: autoimmunity in response to food antigen. Semin Immunol 27(5):343–352
Fasano A, Berti I, Gerarduzzi T et al (2003) Prevalence of celiac disease in at-risk and not-at-risk groups in the United States: a large multicenter study. Arch Intern Med 163(3):286–292
Lionetti E, Gatti S, Pulvirenti A et al (2015) Celiac disease from a global perspective. Best Pract Res Clin Gastroenterol 29(3):365–379
Catassi C, Bai JC, Bonaz B et al (2013) Non-celiac gluten sensitivity: the new frontier of gluten related disorders. Nutrients 5(10):3839–3853
Koerner TB, Abbott M, Godefroy SB et al (2013) Validation procedures for quantitative gluten ELISA methods: AOAC allergen community guidance and best practices. J AOAC Int 96(5):1033–1040
Thompson T, Mendez E (2008) Commercial assays to assess gluten content of gluten-free foods: why they are not created equal. J Am Diet Assoc 108(10):1682–1687
Colgrave ML, Byrne K, Blundell M et al (2016) Identification of barley-specific peptide markers that persist in processed foods and are capable of detecting barley contamination by LC-MS/MS. J Proteome 147:169–176
Colgrave ML, Goswami H, Byrne K et al (2015) Proteomic profiling of 16 cereal grains and the application of targeted proteomics to detect wheat contamination. J Proteome Res 14(6):2659–2668
Fiedler KL, McGrath SC, Callahan JH et al (2014) Characterization of grain-specific peptide markers for the detection of gluten by mass spectrometry. J Agric Food Chem 62(25):5835–5844
Gomaa A, Boye J (2015) Simultaneous detection of multi-allergens in an incurred food matrix using ELISA, multiplex flow cytometry and liquid chromatography mass spectrometry (LC-MS). Food Chem 175:585–592
Sealey-Voyksner JA, Khosla C, Voyksner RD et al (2010) Novel aspects of quantitation of immunogenic wheat gluten peptides by liquid chromatography-mass spectrometry/mass spectrometry. J Chromatogr A 1217(25):4167–4183
Acknowledgments
This work was supported by a fellowship to HL “International Training for High-level Talent in 2016” (YUWAIZHUAN [2016] No.8) from Foreign Experts Bureau of Henan Province.
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2019 Springer Science+Business Media, LLC, part of Springer Nature
About this protocol
Cite this protocol
Li, H., Byrne, K., Howitt, C.A., Colgrave, M.L. (2019). Efficient Extraction and Digestion of Gluten Proteins. In: Wang, X., Kuruc, M. (eds) Functional Proteomics. Methods in Molecular Biology, vol 1871. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8814-3_22
Download citation
DOI: https://doi.org/10.1007/978-1-4939-8814-3_22
Published:
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-8813-6
Online ISBN: 978-1-4939-8814-3
eBook Packages: Springer Protocols