Abstract
To achieve the most accurate assessment of functional Ca2+ channel or modulator properties and their regulation, a patch clamp technique to record membrane currents is required. This technique has wide applications ranging from recording the activity of native channels in their natural environment to that of recombinant channels expressed in heterologous cells. This chapter introduces the methods that have been used for the detection of calcium release-activated calcium (CRAC) currents, one of the store-operated calcium entry pathways, in human primary T cells. This standard protocol is for laboratories already equipped with a full patch clamp setup or for investigators collaborating with laboratories experienced in patch clamp.
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References
Molleman A (2002) Patch clamping: an introductory guide to patch clamp electrophysiology. John Wiley & Sons, Ltd., Chichester
Liu S, Watanabe S, Shudou M, Kuno M, Miura H, Maeyama K (2014) Upregulation of store-operated Ca entry in the naive CD4 T cells with aberrant cytokine releasing in active rheumatoid arthritis. Immunol Cell Biol 92:752–760
Thakur P, Fomina AF (2010) Whole-cell recording of calcium release-activated calcium (CRAC) currents in human T lymphocytes. J Vis Exp (46):e2346
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Liu, S., Kuno, M. (2018). Electrophysiological Methods to Measure Ca2+ Current. In: Liu, S. (eds) Rheumatoid Arthritis. Methods in Molecular Biology, vol 1868. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8802-0_18
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DOI: https://doi.org/10.1007/978-1-4939-8802-0_18
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-8801-3
Online ISBN: 978-1-4939-8802-0
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