Abstract
Double-stranded RNA (dsRNA) molecules are widely found in yeasts and filamentous fungi. It has been suggested that these molecules may play an important role in the evolution of eukaryote genomes and could be a valuable tool in yeast typing. The characterization of these extrachromosomal genetic elements is usually a laborious process, especially when trying to analyze a large number of samples. In this chapter, we describe a simple method to isolate dsRNA elements from yeasts using low amounts of starting material and their application to different Xanthophyllomyces dendrorhous strains and other psychrotolerant carotenogenic yeasts. Furthermore, the methodologies for enzymatic and hybridization characterizations and quantification of relative dsRNA abundance are detailed.
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Acknowledgments
This work was supported by grants FONDECYT 11060157 and 1130333, Innova CORFO 07CN13PZT-17, and INACH RT_07-13.
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Baeza, M., Fernández-Lobato, M., Alcaíno, J., Cifuentes, V. (2018). Isolation and Characterization of Extrachromosomal Double-Stranded RNA Elements from Carotenogenic Yeasts. In: Barreiro, C., Barredo, JL. (eds) Microbial Carotenoids. Methods in Molecular Biology, vol 1852. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8742-9_20
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DOI: https://doi.org/10.1007/978-1-4939-8742-9_20
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