Abstract
OMICs-based investigations of microorganisms are becoming more and more widespread in the upcoming era of systems and synthetic biology. Here, proteomics plays a key role and two-dimensional difference gel electrophoresis (2D DIGE) remains the “gold-standard” for globally determining protein abundance changes on a quantitative and statistically confident level—in particular also for laboratories not having full-cycle proteomic facilities at their disposal. In this contribution we summarize our methodological procedures and experiences with 2D DIGE accumulated over the past 15 years.
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Acknowledgment
Proteomic research (incl. DIGE) in our laboratory was supported by the German Federal Ministry of Education and Research BMBF (REGX-project), the Max Planck Society, and the Deutsche Forschungsgemeinschaft (SPP 1319, SFB TRR 51, GRK 1885).
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Trautwein, K., Rabus, R. (2018). Applications of Difference Gel Electrophoresis (DIGE) in the Study of Microorganisms. In: Becher, D. (eds) Microbial Proteomics. Methods in Molecular Biology, vol 1841. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8695-8_8
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DOI: https://doi.org/10.1007/978-1-4939-8695-8_8
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