Abstract
The induction of retinal degeneration by light exposure is widely used to study mechanisms of cell death. The advantage of such light-induced lesions over genetically determined degenerations is that light exposures can be manipulated according to the needs of the experimenter. Bright white light exposure can induce a synchronized burst of apoptosis in photoreceptors in a large retinal area which permits to study cellular and molecular events in a controlled fashion. Blue light of high energy induces a hot spot of high retinal irradiance within very short exposure durations (seconds to minutes) and may help to unravel the initial events after light absorption which may be similar for all damage regimens. These initial events may then induce various molecular signaling pathways and secondary effects such as lipid and protein oxidation, which may be varying in different light damage setups and different strains or species, respectively. Blue light lesions also allow to study cellular responses in a circumscribed retinal area (hot spot) in comparison with the surrounding tissue.
Here we describe the methods for short-term exposures (within the hours range) to bright full-spectrum white light and for short exposures (seconds to minutes) to high-energy monochromatic blue or green light.
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References
Samardzija M, Neuhauss SCF, Joly S, Kurz-Levin M, Grimm C (2010) Animal models for retinal degeneration. In: Pang I-H, Clark AF (eds) Advances in experimental medicine and biology, Retinal degenerative diseases. Humana Press, Springer Science+Business Media, Germany, pp 52–80
Hao W, Wenzel A, Obin MS, Chen CK, Brill E, Krasnoperova NV, Eversole-Cire P, Kleyner Y, Taylor A, Simon MI, Grimm C, Reme CE, Lem J (2002) Evidence for two apoptotic pathways in light-induced retinal degeneration. Nat Genet 32:254–260
van Norren D, Gorgels TG (2011) The action spectrum of photochemical damage to the retina: a review of monochromatic threshold data. Photochem Photobiol 87:747–753
Grimm C, Wenzel A, Groszer M, Mayser H, Seeliger M, Samardzija M, Bauer C, Gassmann M, Reme CE (2002) HIF-1-induced erythropoietin in the hypoxic retina protects against light-induced retinal degeneration. Nat Med 8:718–724
Organisciak DT, Darrow RM, Barsalou L, Kutty RK, Wiggert B (2000) Circadian-dependent retinal light damage in rats. Invest Ophthalmol Vis Sci 41:3694–3701
Joly S, Francke M, Ulbricht E, Beck S, Seeliger M, Hirrlinger P, Hirrlinger J, Lang KS, Zinkernagel M, Odermatt B, Samardzija M, Reichenbach A, Grimm C, Remé CE (2009) Cooperative phagocytes. Resident microglia and bone marrow immigrants remove dead photoreceptors in retinal lesions. Am J Pathol 174:2310–2322
Breton ME, Schueller AW, Lamb TD, Pugh EN Jr (1994) Analysis of ERG a-wave amplification and kinetics in terms of the G-protein cascade of phototransduction. Invest Ophthalmol Vis Sci 35:295–309
Grimm C, Remé CE, Rol PO, Williams TP (2000) Blue light’s effects on rhodopsin: photoreversal of bleaching in living rat eyes. Invest Ophthalmol Vis Sci 41:3983–3990
Grimm C, Wenzel A, Williams TP, Rol PO, Hafezi F, Remé CE (2001) Rhodopsin - mediated blue-light damage to the rat retina: effect of photoreversal of bleaching. Invest Ophthalmol Vis Sci 42:497–505
Wenzel A, Grimm C, Samardzija M, Reme CE (2003) The genetic modifier Rpe65Leu(450): effect on light damage susceptibility in c-Fos-deficient mice. Invest Ophthalmol Vis Sci 44:2798–2802
Wenzel A, Grimm C, Seeliger MW, Jaissle G, Hafezi F, Kretschmer R, Zrenner E, Reme CE (2001) Prevention of photoreceptor apoptosis by activation of the glucocorticoid receptor. Invest Ophthalmol Vis Sci 42:1653–1659
Keller C, Grimm C, Wenzel A, Hafezi F, Remé CE (2001) Protective effect of halothane anesthesia on retinal light damage: inhibition of metabolic rhodopsin regeneration. Invest Ophthalmol Vis Sci 42:476–480
Acknowledgments
We thank the late Theodore P. Williams for numerous stimulating discussions, helping with setting up the light damage systems, analyzing data, and providing crucial insights into the function and metabolism of rhodopsin.
We also cordially thank François Delori and Ed Pugh Jr. for contributing to the blue light damage setup by calculating retinal irradiance, photon flux, and rate of rhodopsin isomerizations.
The late Pascal Rol helped to build the first blue light setup, which was later refined by Michael Mrochen and Thomas Menzi.
Discussions with and ideas from all past and present members of the Lab for Retinal Cell Biology helped to improve the light exposure systems. Without their input and experiments, many of the potential pitfalls would not have been recognized and eliminated.
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Grimm, C., Remé, C.E. (2019). Light Damage Models of Retinal Degeneration. In: Weber, B.H.F., Langmann, T. (eds) Retinal Degeneration. Methods in Molecular Biology, vol 1834. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-8669-9_12
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DOI: https://doi.org/10.1007/978-1-4939-8669-9_12
Publisher Name: Humana, New York, NY
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