Abstract
Variable fragments of heavy-chain-only antibodies (VHH) found in camelids are valuable research tools in pharmacology and biotechnology and are being developed for the clinic to treat patients with autoimmune and infectious diseases or cancer. Their single-domain nature and biochemical properties greatly facilitate the development process. The most common technology to select single-domain antibody fragments is phage display following active immunization of llamas or other members of Camelidae family. Selection of VHH from immune phage libraries is a rapid approach to discover a broad panel of in vivo matured antigen-specific clones with comprehensive functionalities. In this chapter, we describe a detailed protocol for construction of VHH immune libraries and phage display selection against antigens in their native conformation.
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Acknowledgments
We thank Maria Gonzalez Pajuelo and Fair Journey Biologics for providing the figure of VHH selection and helping in writing this manuscript. This work has been funded by European Union’s Horizon2020 MSCA Program under grant agreement 641833 (ONCORNET).
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Bobkov, V., van der Woning, B., de Haard, H. (2018). Display Technologies for Generation of Ig Single Variable Domains. In: Nevoltris, D., Chames, P. (eds) Antibody Engineering. Methods in Molecular Biology, vol 1827. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8648-4_7
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DOI: https://doi.org/10.1007/978-1-4939-8648-4_7
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