Abstract
Noble metal nanoparticles are well known for their unique optical properties. Density oscillations of the nanoparticle conduction electrons are induced at a specific frequency by an external incident light beam. This phenomenon is known under the term localized surface plasmon resonance (LSPR). The spectral position of the resonance band is determined by shape, size, and material of the nanoparticle and influenced by changes of the local refractive index of the surrounding medium. The latter gives the opportunity to use noble metal nanoparticles as label-free bioanalytical sensors. Biomolecules can be bound directly on the nanoparticle surface, which leads to a change of the local refractive index, and a shift of the peak maximum is detected by absorbance spectroscopy. This method is used for bioanalytical diagnostics. Here, a DNA sensing protocol for real-time measurements in situ using this system will be presented. A dense layer of noble metal nanoparticles is immobilized on a glass substrate and implemented in a microfluidic chamber, where the spectroscopic measurements are conducted.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Similar content being viewed by others
References
Kreibig U, Vollmer M (1995) Optical properties of metal clusters, Series in materials science, vol 25. Springer, Berlin
Schneider T, Jahr N, Jatschka J, Csaki A, Stranik O, Fritzsche W (2013) Localized surface plasmon resonance (LSPR) study of DNA hybridization at single nanoparticle transducers. J Nanopart Res 15(4):1–10. https://doi.org/10.1007/s11051-013-1531-7
Jatschka J, Dathe A, Csáki A, Fritzsche W, Stranik O (2016) Propagating and localized surface plasmon resonance sensing—a critical comparison based on measurements and theory. Sens Bio-Sensing Res 7:62–70. https://doi.org/10.1016/j.sbsr.2016.01.003
Dahlin AB, Tegenfeldt JO, Höök F (2006) Improving the instrumental resolution of sensors based on localized surface Plasmon resonance. Anal Chem 78(13):4416–4423. https://doi.org/10.1021/ac0601967
Zhang X, Servos MR, Liu J (2012) Instantaneous and quantitative functionalization of gold nanoparticles with thiolated DNA using a pH-assisted and surfactant-free route. J Am Chem Soc 134(17):7266–7269. https://doi.org/10.1021/ja3014055
Herne TM, Tarlov MJ (1997) Characterisation of DNA probes immobilized on gold surfaces. J Am Chem Soc 119:8916–8920
Levitcky R, Herne TM, Tarlov MJ, Satija SK (1998) Using self-assembly to control the structure of DNA monolayers on gold: a neutron reflectivity study. J Am Chem Soc 120:9787–9792
Dupont-Filliard A, Billon M, Livache T, Guillerez S (2004) Biotin/avidin system for the generation of fully renewable DNA sensor based on biotinylated polypyrrole film. Anal Chim Acta 515(2):271–277. https://doi.org/10.1016/j.aca.2004.03.072
Fang Y, Hoh JH (1998) Surface-directed DNA condensation in the absence of soluble multivalent cations. Nucleic Acids Res 26(2):588–593
Csáki A, Jahn F, Latka I, Henkel T, Malsch D, Schneider T, Schröder K, Schuster K, Schwuchow A, Spittel R, Zopf D, Fritzsche W (2010) Nanoparticle layer deposition for plasmonic tuning of microstructured optical fibers. Small 6(22):2584–2589. https://doi.org/10.1002/smll.201001071
Acknowledgments
We thankfully acknowledge Jacqueline Jatschka for further measurements as well as Andrè Dathe and Ondrej Stranik for the data recording script and the setup. The funding of ExoDiagnos (EU Era-NET-AiFZIM KF2206925CR4) and TRACE (EU Era-NET-KIT 02WU1348A) is gratefully acknowledged.
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2018 Springer Science+Business Media, LLC, part of Springer Nature
About this protocol
Cite this protocol
Thamm, S., Csàki, A., Fritzsche, W. (2018). LSPR Detection of Nucleic Acids on Nanoparticle Monolayers. In: Zuccheri, G. (eds) DNA Nanotechnology. Methods in Molecular Biology, vol 1811. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8582-1_11
Download citation
DOI: https://doi.org/10.1007/978-1-4939-8582-1_11
Published:
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-8581-4
Online ISBN: 978-1-4939-8582-1
eBook Packages: Springer Protocols