Abstract
We present a UPLC®-High Resolution Mass Spectrometric method to simultaneously screen for 19 benzodiazepines, 12 opiates, cocaine and three metabolites, and 3 “Z-drug” hypnotic sedatives in both blood and urine specimens. Sample processing consists of a high-speed, high-temperature enzymatic hydrolysis for urine samples followed by a rapid supported liquid extraction (SLE). The combination of ultrahigh-resolution chromatography with high resolution mass spectrometry allows all analytes to be uniquely detected with a 10 min analytical run. Limits of detection for all target analytes are 3 ng/mL or better, with only 300 μL of specimen used for analysis. The combination of low sample volume with fast processing and analysis makes this method a suitable replacement for immunoassay screening of the targeted drug classes, while providing far superior specificity and better limits of detection than can routinely be obtained by immunoassay.
This is a publication of the Laboratory Division of the Federal Bureau of Investigation (FBI). Names of commercial manufacturers are provided for identification purposes only, and inclusion does not imply endorsement by the FBI. The views expressed in this article are those of the authors and do not necessarily reflect the official policy or position of the FBI or the U.S. Government. This work was prepared as part of their official duties. Title 17 U.S.C. 105 provides that ‘Copyright protection under this title is not available for any work of the United States Government’. Title 17 U.S.C. 101 defines a United States Government work as a work prepared by an employee of the United States Government as part of that person’s official duties.
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Jagerdeo, E., Schaff, J.E. (2018). UPLC-Orbitrap® Screening for over 35 Drugs of Abuse and Metabolites in Biological Fluids in Under 10 min. In: Musah, R. (eds) Analysis of Drugs of Abuse. Methods in Molecular Biology, vol 1810. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8579-1_7
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DOI: https://doi.org/10.1007/978-1-4939-8579-1_7
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