Abstract
During the last 50 years, microdialysis technique has been continuously improved to become a well-established method to monitor local concentrations of neurotransmitters. In respect to other currently used techniques, such as voltammetry, microdialysis has the advantage to be possibly applied to all measurable neurotransmitters and to allow local treatments. These properties render the technique a suitable approach to investigate, in vivo, the neurochemical consequences of receptor–receptor interactions, thus providing functional correlates to binding and other biochemical data.
This chapter is mainly focused on a general description of microdialysis technique in freely moving animals and on the application of one- or dual-probe(s) microdialysis to investigate the functional relevance of receptor–receptor interactions in rodent brain.
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References
Chefer VI, Thompson AC, Zapata A et al (2009) Overview of brain microdialysis. Curr Protoc Neurosci Chapter 7:Unit7.1
Pan YF, Feng J, Cheng QY et al (2007) Intracerebral microdialysis technique and its application on brain pharmacokinetic-pharmacodynamic study. Arch Pharm Res 30:1635–1645
Höcht C, Opezzo JA, Taira CA (2007) Applicability of reverse microdialysis in pharmacological and toxicological studies. J Pharmacol Toxicol Methods 5:3–15
Hutchinson PJ, Jalloh I, Helmy A et al (2015) Consensus statement from the 2014 International Microdialysis Forum. Intensive Care Med 41:1517–1528
Bito L, Davson H, Levin E et al (1966) The concentration of free amino acids and other electrolytes in cerebrospinal fluid: In vivo dialysis of brain and blood plasma of the dog. J Neurochem 13:1057–1067
Delgado JMR, DeFeudis FV, Roth RH et al (1974) Dialytrode for long term intracerebral perfusion in awake monkeys. Arch Int Pharmacodyn 198:9–21
Ungerstedt U, Pycock C (1974) Functional correlates of dopamine neurotransmission. Bull Schweiz Akad Med Wiss 30:44–55
Westerink BHC, Justice JB Jr (1991) Microdialysis compared with other in vivo release models. In: Robinson TE, Justice JB Jr (eds) Microdialysis in the Neurosciences. Elsevier Science Publishing, New York, pp 23–43
Ungerstedt U (1991) Microdialysis--principles and applications for studies in animals and man. J Intern Med 230:365–373
Thompson AC, Shippenberg TS (2001) Microdialysis in rodents. Curr Protoc Neurosci Chapter 7:Unit7.2
Anderzhanova E, Wotjak CT (2013) Brain microdialysis and its applications in experimental neurochemistry. Cell Tissue Res 354:27–39
König M, Thinnes A, Klein J (2017) Microdialysis and its use in behavioural studies: focus on acetylcholine. J Neurosci Methods S0165-0270(17):30294–30297
Benveniste H, Drejer J, Schousboe A et al (1987) Regional cerebral glucose phosphorylation and blood flow after insertion of a microdialysis fiber through the dorsal hippocampus in the rat. J Neurochem 49:729–734
Benveniste H, Diemer NH (1987) Cellular reactions to implantation of a microdialysis tube in the rat hippocampus. Acta Neuropathol 74:234–238
Georgieva J, Luthman J, Mohringe B et al (1993) Tissue and microdialysate changes after repeated and permanent probe implantation in the striatum of freely moving rats. Brain Res Bull 31:463–470
Zapata A, Chefer VI, Shippenberg TS (2009) Microdialysis in rodents. Curr Protoc Neurosci Chapter 7:Unit7.2
Herrera-Marschitz M, Meana JJ, O'Connor WT et al (1992) Neuronal dependence of extracellular dopamine, acetylcholine, glutamate, aspartate and gamma-aminobutyric acid (GABA) measured simultaneously from rat neostriatum using in vivo microdialysis: reciprocal interactions. Amino Acids 2:157–179
Morari M, O'Connor WT, Darvelid M et al (1996) Functional neuroanatomy of the nigrostriatal and striatonigral pathways as studied with dual probe microdialysis in the awake rat–I. Effects of perfusion with tetrodotoxin and low-calcium medium. Neuroscience 72:79–87
LaLumiere RT, Kalivas PW (2008) Glutamate release in the nucleus accumbens core is necessary for heroin seeking. J Neurosci 28:3170–3177
Fuxe K, Canals M, Torvinen M et al (2007) Intramembrane receptor-receptor interactions: a novel principle in molecular medicine. J Neural Transm (Vienna) 114:49–75
Hernández L, Paredes D, Rada P (2011) Feeding behavior as seen through the prism of brain microdialysis. Physiol Behav 104:47–56
Lietsche J, Gorka J, Hardt S et al (2015) Custom-made Microdialysis Probe Design. J Vis Exp 101:e53048
Paxinos G, Watson C (1986) The rat brain in stereotaxic coordinates. Academic, New York
Ferraro L, O'Connor WT, Antonelli T et al (1997) Differential effects of intrastriatal neurotensin(1-13) and neurotensin(8-13) on striatal dopamine and pallidal GABA release. A dual-probe microdialysis study in the awake rat. Eur J Neurosci 9:1838–1846
Ferraro L, O’Connor WT, Beggiato S et al (2012) Striatal NTS1, dopamine D2 and NMDA receptor regulation of pallidal GABA and glutamate release–a dual-probe microdialysis study in the intranigral 6-hydroxydopamine unilaterally lesioned rat. Eur J Neurosci 35:207–220
Alexander GE, Crutcher MD (1990) Functional architecture of basal ganglia circuits: neural substrates of parallel processing. Trends Neurosci 13:266–271
Chevalier G, Deniau JM (1990) Disinhibition as a basic process in the expression of striatal functions. Trends Neurosci 13:277–280
Quirion R, Chiueh CC, Everist HD et al (1985) Comparative localization of neurotensin receptors on nigrostriatal and mesolimbic dopaminergic terminals. Brain Res 327:385–389
Fuxe K, O’Connor WT, Antonelli T et al (1992) Evidence for a substrate of neuronal plasticity based on pre- and postsynaptic neurotensin-dopamine receptor interactions in the neostriatum. Proc Natl Acad Sci U S A 89:5591–5595
Agnati LF, Fuxe K, Benfenati F et al (1983) Neurotensin in vitro markedly reduces the affinity in subcortical limbic [3H]N-propylnorapomorphine binding sites. Acta Physiol Scand 117:299–301
von Euler G, Fuxe K, Benfenati F et al (1987) Neurotensin modulates the binding characteristics of dopamine D2 receptors in rat striatal membranes following treatment with toluene. Acta Physiol Scand 135:442–448
Fuxe K, Agnati LF, von Euler G (1992) Neuropeptides, excitatory amino acid and adenosine A2 receptors regulate D2 receptors via intramembrane receptor-receptor interactions. Relevance for Parkinson’s disease and schizophrenia. Neurochem Int 20:215S–224S
Gully D, Canton M, Boigegrain R et al (1993) Biochemical and pharmacological profile of a potent and selective nonpeptide antagonist of the neurotensin receptor. Proc Natl Acad Sci U S A 90:65–69
Reid MS, O'Connor WT, Herrera-Marschitz M et al (1990) The effects of intranigral GABA and dynorphin A injections on striatal dopamine and GABA release: evidence that dopamine provides inhibitory regulation of striatal GABA neurons via D2 receptors. Brain Res 519:255–260
Granier C, van Rietschoten J, Kitabgi P et al (1982) Synthesis and characterization of neurotensin analogues for structure/activity relationship studies. Acetyl-neurotensin-(8-13) is the shortest analogue with full binding and pharmacological activities. Eur J Biochem 124:117–24
Sirinathsinghji DJ, Heavens RP (1989) Stimulation of GABA release from the rat neostriatum and globus pallidus in vivo by corticotropin-releasing factor. Neurosci Lett 100:203–209
Parent A, Hazrati LN (1995) Functional anatomy of the basal ganglia. I. The cortico-basal ganglia-thalamo-cortical loop. Brain Res Brain Res Rev 20:91–127
Ferraro L, Tomasini MC, Fernandez M et al (2001) Nigral neurotensin receptor regulation of nigral glutamate and nigroventral thalamic GABA transmission: a dual-probe microdialysis study in intact conscious rat brain. Neuroscience 102:113–120
Antonelli T, Tomasini MC, Fuxe K et al (2007) Focus on NTR/D2 interactions in the basal ganglia. J Neural Transm (Vienna) 114:105–113
Guidolin D, Agnati LF, Marcoli M et al (2015) G-protein-coupled receptor type A heteromers as an emerging therapeutic target. Expert Opin Ther Targets 19:265–283
Kennedy RT (2013) Emerging trends in in vivo neurochemical monitoring by microdialysis. Curr Opin Chem Biol 17:860–867
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Tanganelli, S. et al. (2018). In Vivo Microdialysis Technique Applications to Understand the Contribution of Receptor–Receptor Interactions to the Central Nervous System Signaling. In: FUXE, K., Borroto-Escuela, D. (eds) Receptor-Receptor Interactions in the Central Nervous System. Neuromethods, vol 140. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8576-0_7
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DOI: https://doi.org/10.1007/978-1-4939-8576-0_7
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