Abstract
Schizophrenia is a devastating mental disorder, affecting almost 1% of the world population, and has a tremendous effect on social and occupational functioning. Dopamine D2 receptors (D2Rs) are the main targets of typical antipsychotic medications for schizophrenia, where they can effectively alleviate the positive symptoms by antagonizing D2Rs. Thus, investigating different modulation of D2R function is important in identifying novel drug targets and therapeutics for better outcomes in schizophrenia. Protein–protein interactions between D2Rs and other proteins are critical in regulating D2R signaling and subsequent downstream physiological functions. Here we described various biochemical methods including co-immunoprecipitation and protein affinity purification assays, that are commonly used to characterize D2R-associated protein complexes. Specifically, we reviewed the D2R–D1R and D2R–DISC1 interactions and discussed their association in the pathophysiology of schizophrenia. This chapter aims to provide systemic guidelines for the standard biochemical techniques in identifying D2R-associated protein–protein interactions, and to investigate the roles of these interactions in the brain.
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Su, P., Lee, F.H.F., Liu, F. (2018). Biochemical Characterization of Dopamine D2 Receptor-Associated Protein Complexes Using Co-Immunoprecipitation and Protein Affinity Purification Assays. In: FUXE, K., Borroto-Escuela, D. (eds) Receptor-Receptor Interactions in the Central Nervous System. Neuromethods, vol 140. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8576-0_11
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DOI: https://doi.org/10.1007/978-1-4939-8576-0_11
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