Abstract
The gas exchange surface of the lungs is lined by an epithelium consisting of alveolar type (AT) I and ATII cells. ATII cells function to produce surfactant, play a role in host defense and fluid and ion transport, and serve as progenitors. ATI cells are important for gas exchange and fluid and ion transport. Our understanding of the biology of these cells depends on the investigation of isolated cells. Here, we present methods for the isolation of mouse and rat ATII cells.
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Notes
- 1.
Not used by Borok lab
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Acknowledgments
This work was supported by NIH HL131608 (RLZ), the Boettcher Foundation (RLZ), and funds from the University of Colorado Denver Department of Medicine Outstanding Early Career Scholars Program (RLZ) and the Hastings Foundation (ZB).
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Jansing, N.L. et al. (2018). Isolation of Rat and Mouse Alveolar Type II Epithelial Cells. In: Alper, S., Janssen, W. (eds) Lung Innate Immunity and Inflammation. Methods in Molecular Biology, vol 1809. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8570-8_6
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DOI: https://doi.org/10.1007/978-1-4939-8570-8_6
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Publisher Name: Humana Press, New York, NY
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Online ISBN: 978-1-4939-8570-8
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